mgr Kamil Krakowski

@article{Korsak2025,

title = {Characterization of Cronobacter spp. isolated from food products in Poland and comparative genomic analysis of Cronobacter sakazakii isolate MK_10 and a clinical strain associated with a fatal neonatal infection},

author = {Dorota Korsak and Magdalena Szuplewska and Paulina Kozłowska and Kamil Krakowski and Cora Chmielowska and Paweł Wawrzyniak and Elżbieta Maćkiw and Dariusz Bartosik},

doi = {10.3389/fmicb.2025.1713963},

issn = {1664-302X},

year  = {2025},

date = {2025-12-19},

urldate = {2025-12-19},

journal = {Front. Microbiol.},

volume = {16},

publisher = {Frontiers Media SA},

abstract = {<jats:p>

                    <jats:italic>Cronobacter</jats:italic>

                    spp. are ubiquitous environmental bacteria associated with infections affecting all age groups, with infants and elderly individuals particularly at risk. We investigated the prevalence of

                    <jats:italic>Cronobacter</jats:italic>

                    spp. in 251 food samples collected in Poland, including raw milk, powdered milk, infant formula, dried herbs and spices, dried teas, and raw vegetables.

                    <jats:italic>Cronobacter</jats:italic>

                    strains were isolated from 26 samples (10.4%) and classified into five species:

                    <jats:italic>C. sakazakii</jats:italic>

                    ,

                    <jats:italic>C. muytjensii</jats:italic>

                    ,

                    <jats:italic>C. turicensis</jats:italic>

                    ,

                    <jats:italic>C. malonaticus</jats:italic>

                    and

                    <jats:italic>C. dublinensis</jats:italic>

                    . The isolates were grouped based on sequence variation of the

                    <jats:italic>fusA</jats:italic>

                    and

                    <jats:italic>rpoB</jats:italic>

                    genes, and further differentiated according to their biochemical profiles. The complete nucleotide sequence of

                    <jats:italic>C. sakazakii</jats:italic>

                    MK_10, a plasmid-rich strain isolated from dried oregano and representing the most prevalent species in the collected strain pool, was obtained. Comparative genomic analysis of MK_10, the first fully sequenced foodborne

                    <jats:italic>C. sakazakii</jats:italic>

                    strain in Poland, with a clinical strain from a fatal neonatal case revealed the presence of a conserved set of genes associated with pathogenic properties. MK_10 carries three extrachromosomal replicons, including two IncFIB plasmids – pCS-MK10_P1 (114 kb), representing the pESA3 family of virulence plasmids, and pCS-MK10_P2 (45.9 kb) carrying a copper homeostasis and silver resistance island (CHASRI). Comparative analyses revealed that the structure of the MK_10 chromosome has been shaped by multiple insertion and deletion events, associated mainly with prophages (e.g., a novel intact prophage related to

                    <jats:italic>Cronobacter</jats:italic>

                    phage ENT47670) and other integrative elements. The MK_10 genome contains only two transposable elements: an isoform of insertion sequence IS

                    <jats:italic>Ehe2</jats:italic>

                    (IS

                    <jats:italic>3</jats:italic>

                    family) and a non-autonomous Tn

                    <jats:italic>3</jats:italic>

                    -family transposon of unique structure – both located within two functional integrative elements (IE), designated IE

                    <jats:italic>

                      Csak

                      <jats:sub>MK10</jats:sub>

                      1

                    </jats:italic>

                    (32.3 kb) and IE

                    <jats:italic>

                      Csak

                      <jats:sub>MK10</jats:sub>

                      2

                    </jats:italic>

                    (32.4 kb). The identified mobile genetic elements, plasmids, and IEs, harbor genes of adaptive value, which potentially contribute to the strain’s environmental fitness and pathogenic potential.

                  </jats:p>},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{KKr1,

title = {RNA-Targeting CRISPR–Cas Systems and Their Applications},

author = {Michal Burmistrz and Kamil Krakowski and Agata Krawczyk-Balska},

doi = {10.3390/ijms21031122},

issn = {1422-0067},

year  = {2020},

date = {2020-01-01},

journal = {International Journal of Molecular Sciences},

volume = {21},

pages = {1122},

abstract = {Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) systems have revolutionized modern molecular biology. Numerous types of these systems have been discovered to date. Many CRISPR–Cas systems have been used as a backbone for the development of potent research tools, with Cas9 being the most widespread. While most of the utilized systems are DNA-targeting, recently more and more attention is being gained by those that target RNA. Their ability to specifically recognize a given RNA sequence in an easily programmable way makes them ideal candidates for developing new research tools. In this review we summarize current knowledge on CRISPR–Cas systems which have been shown to target RNA molecules, that is type III (Csm/Cmr), type VI (Cas13), and type II (Cas9). We also present a list of available technologies based on these systems.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}