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Molecular Biology of Euglenids

 

Research topics

The research in our team focuses mostly on the three current research projects financed by the Science Centre Poland. As part of the first project we analyse the nuclear genomes of two euglenid species – Euglena longa and Euglena hiemalis – focusing on the existing introns of various types (their spacing, potential changes of the speed and the mechanism of gaining new intron sequences). The aim of the second project is the characteristics of the nonconventional introns, unique to euglenids. We are attempting to: confirm the physical form in which they are removed, the removal order of introns of various types from the transcripts, learn the localisation in the cell of the nonconventional splicing, affix the mechanism of gaining new introns in genes as well as the removal mechanism during the pre-mRNA maturation. The third projects concern the analysis of circular, extrachromosomal DNA molecules. Aside from the ongoing projects, we study the evolution of rRNA coding genes as well as the atypical mitochondrial genome organisation of euglenids.

Research projects

Publications review

Gumińska, Natalia; Zakryś, Bożena; Milanowski, Rafał

A New Type of Circular RNA derived from Nonconventional Introns in Nuclear Genes of Euglenids Journal Article

Journal of Molecular Biology, 433 (3), pp. 166758, 2021.

Abstract | Links | BibTeX

Gumińska, Natalia; Płecha, Magdalena; Walkiewicz, Halszka; Hałakuc, Paweł; Zakryś, Bożena; Milanowski, Rafał

Culture purification and DNA extraction procedures suitable for next-generation sequencing of euglenids Journal Article

Journal of Applied Phycology, 30 (6), pp. 3541–3549, 2018, ISSN: 1573-5176.

Abstract | Links | BibTeX

Gumińska, Natalia; Płecha, Magdalena; Zakryś, Bożena; Milanowski, Rafał

Order of removal of conventional and nonconventional introns from nuclear transcripts of Euglena gracilis Journal Article

PLoS Genetics, 14 (10), pp. e1007761, 2018, ISSN: 15537404.

Abstract | Links | BibTeX

Milanowski, Rafał; Gumińska, Natalia; Karnkowska, Anna; Ishikawa, Takao; Zakryś, Bożena

Intermediate introns in nuclear genes of euglenids – are they a distinct type? Journal Article

BMC Evolutionary Biology, 16 (1), pp. 49, 2016, ISSN: 1471-2148.

Abstract | Links | BibTeX

Milanowski, Rafał; Karnkowska, Anna; Ishikawa, Takao; Zakryś, Bożena

Distribution of Conventional and Nonconventional Introns in Tubulin (alpha and beta) Genes of Euglenids Journal Article

Molecular Biology and Evolution, 31 (3), pp. 584–593, 2014, ISSN: 1537-1719.

Abstract | Links | BibTeX