Muszewska, Anna; Okrasińska, Alicja; Steczkiewicz, Kamil; Drgas, Olga; Orłowska, Małgorzata; Perlińska-Lenart, Urszula; Aleksandrzak-Piekarczyk, Tamara; Szatraj, Katarzyna; Zielenkiewicz, Urszula; Piłsyk, Sebastian; Malc, Ewa; Mieczkowski, Piotr; Kruszewska, Joanna S; Bernat, Przemysław; Pawłowska, Julia Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina Journal Article Frontiers in Microbiology, 12 , pp. 239, 2021. Abstract | Links | BibTeX @article{muszewska2021,
title = {Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina},
author = {Anna Muszewska and Alicja Okrasińska and Kamil Steczkiewicz and Olga Drgas and Małgorzata Orłowska and Urszula Perlińska-Lenart and Tamara Aleksandrzak-Piekarczyk and Katarzyna Szatraj and Urszula Zielenkiewicz and Sebastian Piłsyk and Ewa Malc and Piotr Mieczkowski and Joanna S. Kruszewska and Przemysław Bernat and Julia Pawłowska},
url = {https://www.frontiersin.org/article/10.3389/fmicb.2021.636986},
doi = {10.3389/fmicb.2021.636986},
year = {2021},
date = {2021-02-15},
journal = {Frontiers in Microbiology},
volume = {12},
pages = {239},
abstract = {Mucoromycotina are often considered mainly in pathogenic context but their biology remains understudied. We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e., Umbelopsidales and Mucorales). We selected two Umbelopsis isolates from soil (i.e., U. isabellina, U. vinacea), two soil-derived Mucor isolates (i.e., M. circinatus, M. plumbeus), and two Mucorales representatives with extended proteolytic activity (i.e., Thamnidium elegans and Mucor saturninus). We complement computational genome annotation with experimental characteristics of their digestive capabilities, cell wall carbohydrate composition, and extensive total lipid profiles. These traits inferred from genome composition, e.g., in terms of identified encoded enzymes, are in accordance with experimental results. Finally, we link the presence of associated bacteria with observed characteristics. Thamnidium elegans genome harbors an additional, complete genome of an associated bacterium classified to Paenibacillus sp. This fungus displays multiple altered traits compared to the remaining isolates, regardless of their evolutionary distance. For instance, it has expanded carbon assimilation capabilities, e.g., efficiently degrades carboxylic acids, and has a higher diacylglycerol:triacylglycerol ratio and skewed phospholipid composition which suggests a more rigid cellular membrane. The bacterium can complement the host enzymatic capabilities, alter the fungal metabolism, cell membrane composition but does not change the composition of the cell wall of the fungus. Comparison of early-diverging Umbelopsidales with evolutionary younger Mucorales points at several subtle differences particularly in their carbon source preferences and encoded carbohydrate repertoire. Nevertheless, all tested Mucoromycotina share features including the ability to produce 18:3 gamma-linoleic acid, use TAG as the storage lipid and have fucose as a cell wall component.},
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Mucoromycotina are often considered mainly in pathogenic context but their biology remains understudied. We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e., Umbelopsidales and Mucorales). We selected two Umbelopsis isolates from soil (i.e., U. isabellina, U. vinacea), two soil-derived Mucor isolates (i.e., M. circinatus, M. plumbeus), and two Mucorales representatives with extended proteolytic activity (i.e., Thamnidium elegans and Mucor saturninus). We complement computational genome annotation with experimental characteristics of their digestive capabilities, cell wall carbohydrate composition, and extensive total lipid profiles. These traits inferred from genome composition, e.g., in terms of identified encoded enzymes, are in accordance with experimental results. Finally, we link the presence of associated bacteria with observed characteristics. Thamnidium elegans genome harbors an additional, complete genome of an associated bacterium classified to Paenibacillus sp. This fungus displays multiple altered traits compared to the remaining isolates, regardless of their evolutionary distance. For instance, it has expanded carbon assimilation capabilities, e.g., efficiently degrades carboxylic acids, and has a higher diacylglycerol:triacylglycerol ratio and skewed phospholipid composition which suggests a more rigid cellular membrane. The bacterium can complement the host enzymatic capabilities, alter the fungal metabolism, cell membrane composition but does not change the composition of the cell wall of the fungus. Comparison of early-diverging Umbelopsidales with evolutionary younger Mucorales points at several subtle differences particularly in their carbon source preferences and encoded carbohydrate repertoire. Nevertheless, all tested Mucoromycotina share features including the ability to produce 18:3 gamma-linoleic acid, use TAG as the storage lipid and have fucose as a cell wall component. |
Okrasińska, Alicja; Bokus, Aleksandra; Duk, Katarzyna; Gęsiorska, Aleksandra; Sokołowska, Blanka; Miłobędzka, Aleksandra; Wrzosek, Marta; Pawłowska, Julia New Endohyphal Relationships between Mucoromycota and Burkholderiaceae Representatives Journal Article Applied and Environmental Microbiology, 87 (7), 2021, ISSN: 0099-2240. Abstract | Links | BibTeX @article{Okrasińskae02707-20,
title = {New Endohyphal Relationships between Mucoromycota and Burkholderiaceae Representatives},
author = {Alicja Okrasińska and Aleksandra Bokus and Katarzyna Duk and Aleksandra Gęsiorska and Blanka Sokołowska and Aleksandra Miłobędzka and Marta Wrzosek and Julia Pawłowska},
editor = {Maia Kivisaar},
url = {https://aem.asm.org/content/87/7/e02707-20},
doi = {10.1128/AEM.02707-20},
issn = {0099-2240},
year = {2021},
date = {2021-01-01},
journal = {Applied and Environmental Microbiology},
volume = {87},
number = {7},
publisher = {American Society for Microbiology Journals},
abstract = {Mucoromycota representatives are known to harbor two types of endohyphal bacteria (EHB)—Burkholderia-related endobacteria (BRE) and Mycoplasma-related endobacteria (MRE). While both BRE and MRE occur in fungi representing all subphyla of Mucoromycota, their distribution is not well studied. Therefore, it is difficult to resolve the evolutionary history of these associations in favor of one of the following two alternative hypotheses explaining their origin: textquotedblleftearly invasiontextquotedblright and textquotedblleftlate invasion.textquotedblright Our main goal was to fill this knowledge gap by surveying Mucoromycota fungi for the presence of EHB. We screened 196 fungal strains from 16 genera using a PCR-based approach to detect bacterial 16S rRNA genes, complemented with fluorescence in situ hybridization (FISH) imaging to confirm the presence of bacteria within the hyphae. We detected Burkholderiaceae in ca. 20% of fungal strains. Some of these bacteria clustered phylogenetically with previously described BRE clades, whereas others grouped with free-living Paraburkholderia. Importantly, the latter were detected in Umbelopsidales, which previously were not known to harbor endobacteria. Our results suggest that this group of EHB is recruited from the environment, supporting the late invasion scenario. This pattern complements the early invasion scenario apparent in the BRE clade of EHB.IMPORTANCE Bacteria living within fungal hyphae present an example of one of the most intimate relationships between fungi and bacteria. Even though there are several well-described examples of such partnerships, their prevalence within the fungal kingdom remains unknown. Our study focused on early divergent terrestrial fungi in the phylum Mucoromycota. We found that ca. 20% of the strains tested harbored bacteria from the family Burkholderiaceae. Not only did we confirm the presence of bacteria from previously described endosymbiont clades, we also identified a new group of endohyphal Burkholderiaceae representing the genus Paraburkholderia. We established that more than half of the screened Umbelopsis strains were positive for bacteria from this new group. We also determined that, while previously described BRE codiverged with their fungal hosts, Paraburkholderia symbionts did not.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Mucoromycota representatives are known to harbor two types of endohyphal bacteria (EHB)—Burkholderia-related endobacteria (BRE) and Mycoplasma-related endobacteria (MRE). While both BRE and MRE occur in fungi representing all subphyla of Mucoromycota, their distribution is not well studied. Therefore, it is difficult to resolve the evolutionary history of these associations in favor of one of the following two alternative hypotheses explaining their origin: textquotedblleftearly invasiontextquotedblright and textquotedblleftlate invasion.textquotedblright Our main goal was to fill this knowledge gap by surveying Mucoromycota fungi for the presence of EHB. We screened 196 fungal strains from 16 genera using a PCR-based approach to detect bacterial 16S rRNA genes, complemented with fluorescence in situ hybridization (FISH) imaging to confirm the presence of bacteria within the hyphae. We detected Burkholderiaceae in ca. 20% of fungal strains. Some of these bacteria clustered phylogenetically with previously described BRE clades, whereas others grouped with free-living Paraburkholderia. Importantly, the latter were detected in Umbelopsidales, which previously were not known to harbor endobacteria. Our results suggest that this group of EHB is recruited from the environment, supporting the late invasion scenario. This pattern complements the early invasion scenario apparent in the BRE clade of EHB.IMPORTANCE Bacteria living within fungal hyphae present an example of one of the most intimate relationships between fungi and bacteria. Even though there are several well-described examples of such partnerships, their prevalence within the fungal kingdom remains unknown. Our study focused on early divergent terrestrial fungi in the phylum Mucoromycota. We found that ca. 20% of the strains tested harbored bacteria from the family Burkholderiaceae. Not only did we confirm the presence of bacteria from previously described endosymbiont clades, we also identified a new group of endohyphal Burkholderiaceae representing the genus Paraburkholderia. We established that more than half of the screened Umbelopsis strains were positive for bacteria from this new group. We also determined that, while previously described BRE codiverged with their fungal hosts, Paraburkholderia symbionts did not. |
Pawłowska, Julia; Okrasińska, Alicja; Kisło, Kamil; Aleksandrzak-Piekarczyk, Tamara; Szatraj, Katarzyna; Dolatabadi, Somayeh; Muszewska, Anna Carbon assimilation profiles of mucoralean fungi show their metabolic versatility Journal Article Scientific Reports, 9 , pp. 11864, 2019. Links | BibTeX @article{pawlowska2019,
title = {Carbon assimilation profiles of mucoralean fungi show their metabolic versatility},
author = {Julia Pawłowska and Alicja Okrasińska and Kamil Kisło and Tamara Aleksandrzak-Piekarczyk and Katarzyna Szatraj and Somayeh Dolatabadi and Anna Muszewska },
url = {https://www.nature.com/articles/s41598-019-48296-w#citeas},
doi = {https://doi.org/10.1038/s41598-019-48296-w},
year = {2019},
date = {2019-08-14},
journal = {Scientific Reports},
volume = {9},
pages = {11864},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Banasiak, Łukasz; Pietras, Marcin; Wrzosek, Marta; Okrasińska, Alicja; Gorczak, Michał; Kolanowska, Marta; Pawłowska, Julia Aureoboletus projectellus (Fungi, Boletales) – An American bolete rapidly spreading in Europe as a new model species for studying expansion of macrofungi Journal Article Fungal Ecology, 39 , pp. 94 - 99, 2019, ISSN: 1754-5048. Links | BibTeX @article{BANASIAK201994,
title = {Aureoboletus projectellus (Fungi, Boletales) – An American bolete rapidly spreading in Europe as a new model species for studying expansion of macrofungi},
author = {Łukasz Banasiak and Marcin Pietras and Marta Wrzosek and Alicja Okrasińska and Michał Gorczak and Marta Kolanowska and Julia Pawłowska},
url = {http://www.sciencedirect.com/science/article/pii/S1754504818301491},
doi = {https://doi.org/10.1016/j.funeco.2018.12.006},
issn = {1754-5048},
year = {2019},
date = {2019-01-01},
journal = {Fungal Ecology},
volume = {39},
pages = {94 - 99},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
Pawłowska, Julia; Istel, Łukasz; Gorczak, Michał; Galera, Halina; Wrzosek, Marta; Hawksworth, David L Psychronectria hyperantarctica, gen. nov., comb. nov., epitypification and phylogenetic position of an Antarctic bryophilous ascomycete Journal Article Mycologia, 109 (4), pp. 601—607, 2017, ISSN: 0027-5514. Links | BibTeX @article{PMID:29200362,
title = {Psychronectria hyperantarctica, gen. nov., comb. nov., epitypification and phylogenetic position of an Antarctic bryophilous ascomycete},
author = {Julia Pawłowska and Łukasz Istel and Michał Gorczak and Halina Galera and Marta Wrzosek and David L Hawksworth},
url = {https://doi.org/10.1080/00275514.2017.1398575},
doi = {10.1080/00275514.2017.1398575},
issn = {0027-5514},
year = {2017},
date = {2017-01-01},
journal = {Mycologia},
volume = {109},
number = {4},
pages = {601—607},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
|
