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Evolution of carbon assimilation profiles of Mucorales

Mucorales are evolutionary old lineages of land fungi. They are commonly regarded as ‘sugar fungi’. However, relatively little is known about the ecology and enzymatic abilities of this group. We combined modern molecular biology techniques with high-throughput phenotypic microarrays to determine factors shaping the metabolic abilities of Mucorales.

Main results:

  • 100 Mucorales strains were retrieved from the Westerdijk Fungal Biodiversity Institute’s collection.
  • All strains were maintained in living cultures and comprehensively characterized.
  • The methodology for phenotypic microarrays research was standardized.
  • Assimilation profiles of 95 different carbon sources were obtained on phenotypic microarrays for all strains.
  • A database was developed to gather information on the carbon assimilation profiles of Mucorales.
  • The presence of endohyphal bacteria affecting the ability of fungi to use various substrates was described.
  • It has been shown that clinical strains do not show any specific enzymatic abilities. However, like most members of primary evolutionary lines within Mucorales (Umbelopsidaceae, Saksenaeceae, and Lichtheimiaceae), they are characterized by faster growth on carbohydrates. Thus, it seems that the ability to grow rapidly on various sugars, which was probably an adaptation to rapid colonization of a nutrient-poor terrestrial environment, is currently a factor predisposing some strains to pathogenicity.

Database: download xlsx file

Publications:

  • Pawłowska J., Aleksandrzak-Piekarczyk T., Banach A., Kiersztyn B., Muszewska A., Serewa L., Szatraj K., Wrzosek M. (2016). Preliminary studies on the evolution of carbon assimilation abilities within Mucorales. Fungal Biology 120: 752-763.
  • Li GJ., Hyde KD., Zhao RL., Hongsanan S., Abdel-Aziz FA., Abdel-Wahab MA., Alvarado P., Alves-Silva G., Ammirati JF., Ariyawansa HA., Baghela A., Bahkali AH., Beug M., Bhat DJ., Bojantchev D., Boonpratuang T., Bulgakov TS., Camporesi E., Boro MC., Ceska O., Chakraborty D., Chen JJ., Chethana KWT., Chomnunti P., Consiglio G., Cui BK., Dai DQ., Dai YC., Daranagama DA., Das K., Dayarathne MC., De Crop E., De Oliveira RJV., de Souza CAF., de Souza JI., Dentinger BTM., Dissanayake AJ., Doilom M., Drechsler-Santos ER., Ghobad-Nejhad M., Gilmore SP., Góes-Neto A., Gorczak M., Haitjema CH., Hapuarachchi KK., Hashimoto A., He MQ., Henske JH., Hirayama K., Iribarren MJ., Jayasiri SC., Jayawardena RS., Jeon SJ., Jerônimo GH., Jesus AL., Jones EBG., Kang JC., Karunarathna SC., Kirk PM., Konta S., Kuhnert E., Langer E., Lee HS., Lee HB., Li WJ., Li XH., Liimatainen K., Lima DX., Lin CG., Liu JK., Liu XZ., Liu ZY., Luangsa-ard JJ., Lücking R., Lumbsch HT., Lumyong S., Leaño EM., Marano AV., Matsumura M., McKenzie EHC., Mongkolsamrit S., Mortimer PE., Nguyen TTT., Niskanen T., Norphanphoun C., O’Malley MA., Parnmen S., Pawłowska J., Perera RH., Phookamsak R., Phukhamsakda C., Pires-Zottarelli CLA., Raspé O., Reck MA., Rocha SCO., de Santiago ALCMA., Senanayake IC., Setti L., Shang QJ., Singh S.K., Sir E.B., Solomon K.V., Song J., Srikitikulchai P., Stadler M., Suetrong S., Takahashi H., Takahashi T., Tanaka K., Tang LP., Thambugala KM., Thanakitpipattana D., Theodorou MK., Thongbai B., Thummarukcharoen T., Tian Q., Tibpromma S., Verbeken A., Vizzini A., Vlasák J., Voigt K., Wanasinghe DN., Wang Y., Weerakoon G., Wen HA., Wen TC., Wijayawardene NN., Wongkanoun S., Wrzosek M., Xiao JP., Xu JC., Yan JY., Yang J., Yang SD., Hu Y., Zhang JF., Zhao J., Zhou LW., Peršoh D., Phillips AJL., Maharachchikumbura SSN. (2016). Fungal diversity notes 253–366: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity. 78:1.
  • Pawłowska J., Istel Ł., Gorczak M., Galera H., Wrzosek M., Hawksworth D.L. (2017). Psychronectria hyperantarctica, gen. nov., comb. nov., epitypification and phylogenetic position of an Antarctic bryophilous ascomycete. Mycologia 109(4): 601-607.
  • Hyde K.D., C. Norphanphoun, V.P. Abreu, A. Bazzicalupo, K.W.T. Chethana, M. Clericuzio, M.C. Dayarathne, A.J. Dissanayake, A.H. Ekanayaka, M.-Q. He, S. Hongsanan, S.-K. Huang, S.C. Jayasiri, R.S. Jayawardena, A. Karunarathna, S. Konta, I. Kušan, H. Lee, J. Li, C.-G. Lin, N.-G. Liu, Y.-Z. Lu, Z.-L. Luo, I.S. Manawasinghe, A. Mapook, R.H. Perera, R. Phookamsak, C. Phukhamsakda, I. Siedlecki, A.M. Soares, D.S. Tennakoon, Q. Tian, S. Tibpromma, D.N. Wanasinghe, Y.-P. Xiao, J. Yang, X.-Y. Zeng, F.A. Abdel-Aziz, W.-J. Li, I.C. Senanayake, Q.-J. Shang, D.A. Daranagama, N.I. de Silva, K.M. Thambugala, M.A. Abdel-Wahab, A.H. Bahkali, M.L. Berbee, S. Boonmee, D.J. Bhat, T.S. Bulgakov, B. Buyck, E. Camporesi, R.F. Castañeda-Ruiz, P. Chomnunti, M. Doilom, F. Dovana, T.B. Gibertoni, M. Jadan, R. Jeewon, E.B.G. Jones, J.-C. Kang, S.C. Karunarathna, Y.W. Lim, J.-K. Liu, Z.-Y. Liu, H.L. Plautz Jr., S. Lumyong, S.S.N. Maharachchikumbura, N. Matočec, E.H.C. McKenzie, A. Mešić, D. Miller, J. Pawłowska, O.L. Pereira, I. Promputtha, A.I. Romero, L. Ryvarden, H.-Y. Su, S. Suetrong, Z. Tkalčec, A. Vizzini, T.-C. Wen, K. Wisitrassameewong, M. Wrzosek, J.-C. Xu, Q. Zhao, R.-L. Zhao, P.E. Mortimer (2017). Fungal diversity notes 603–708: taxonomic and phylogenetic notes on genera and species. Fungal Diversity 87(1): 1-235.
  • Wijayawardene NN., Pawłowska J., Letcher P.M., Kirk PM., Humber RA., Schüßler A., Wrzosek M., Muszewska A., Okrasińska A., Istel Ł., Gęsiorska A., Adebola PM., Lateef A., Rajeshkumar KC., Singh RV., Radek R., Walther G., Wagner L., Walker C., Wijesundara DSA., Papizadeh M., Dolatabadi S., Shenoy BD., Tokarev YS., Lumyong S., Hyde KD. (2018). Notes for genera: basal clades of Fungi (including Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Calcarisporiellomycota, Caulochytriomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota, Mucoromycota, Neocallimastigomycota, Olpidiomycota, Rozellomycota and Zoopagomycota). Fungal Diversity 92(1): 43-129
  • Pawłowska J., Okrasińska A., Kisło K., Aleksandrzak-Piekarczyk T., Szatraj K., Dolatabadi S., Muszewska A. (2019). Carbon assimilation profiles of mucoralean fungi show their metabolic versatility. Scientific Reports 9: 11864.