dr Anhelina Kyrychenko

@article{nokey,

title = {Giant DNA Viruses Infecting Unicellular Protists},

author = {A.M. Kyrychenko and V.M. Burkot and I.S. Shcherbatenko},

doi = {10.15407/microbiolj85.04.072},

year  = {2023},

date = {2023-08-16},

urldate = {2023-08-16},

journal = {Microbiological Journal},

volume = {85},

number = {4},

pages = {72—82},

abstract = {Giant viruses (GV) are widespread in various ecosystems and ecological niches of the biosphere, most commonly in marine and freshwater aquatic ecosystems and soils. These viruses infect protists, a paraphyletic group of various unicellular, syncytial, and protozoan multicellular eukaryotes that are not true animals, plants, or fungus. The morphologically and functionally diverse group of protists includes parasites, commensals, or mutualistic symbionts of eukaryots, as well as heterotrophs, autotrophs, and mixotrophs. These giant viruses are currently classified into several families: Mimiviridae, Pithoviridae, Pandoraviridae, Phycodnaviridae, and the Mollivirus genus. GVs of unicellular protists belonging to the Mimiviridae family mainly infect the species of the Acanthamoeba genus. In this review, we provide the available information concerning giant viruses of the Mimiviridae family infecting other protists. These viruses include: Phaeocystis globosa virus PgV-16T (PgV), Aureococcus anophagefferens virus (AaV), Bodo saltans virus (BsV), Chrysochromulina ericina virus (CeV), and Phaeocystis pouchetii virus (PpV), which infect phytoplanktonic protists, as well as a giant virus of microzooplanktonic species, the Cafeteria roenbergensis virus (CroV). The review focuses on the major differences between these viruses and typical objects of current virology, their importance for understanding the evolutionary processes of genomes, genes, proteins, the biosynthetic and defense systems of organisms, as well as the important role of GV in regulating the aquatic microorganisms abundance and species diversity, carbon transfer and nutrient recycling in marine and freshwater reservoirs. Writing this review was motivated by the intention to inspire the interest of scientists in studying viruses as the most widespread biological creatures on Earth and ubiquitous symbiotic partners of all three domains of life.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zabolotny_institute_of_microbiology_and_virology_nas_of_ukraine_weeds_2023,

title = {Weeds as Reservoirs of Viruses in Agrobiocenoses of Cereal Crops in Ukraine},

author = {AM Kyrychenko and MM Bohdan and HO Snihur and IS Shcherbatenko},

url = {https://microbiolj.org.ua/en/archiv/2022-tom-84/6-nov-dec-tom-84/2022-84-6-08/},

doi = {10.15407/microbiolj84.06.072},

issn = {10280987, 26169258},

year  = {2023},

date = {2023-02-01},

urldate = {2023-02-01},

journal = {Mikrobiolohichnyi Zhurnal},

volume = {84},

number = {6},

pages = {72–86},

abstract = {This review examines current knowledge on the prevalence of weeds and feral grass specie as possible reserves of plant viruses in the agroecosystems of Ukraine. Studies concerning mainly virus infection of wheat and barley and weeds distributed in the agrosystems of cereal crops and their impact on virus epidemiology have been summarized in this paper. In addition, great attention is focused on the primary sources of the main causative agents of wheat and barley viral diseases, namely Wheat streak mosaic virus (WSMV), Wheat dwarf virus (WDV), Winter wheat Russian mosaic virus (WWRMV), High Plains wheat mosaic virus (HPWMoV), Barley yellow dwarf virus (BYDV), and Brome mosaic virus (BMV) as well as the main factors contributing to the spread of these viruses in agrocenoses.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{bpl-202301-0008,

title = {Cucumber mosaic virus and turnip mosaic virus occurrence in garlic mustard in Ukraine},

author = {A Kyrychenko and H Snihur and T Schevchenko and I Shcherbatenko and H Korotieieva and O Andriichuk },

url = {https://doi.org/10.32615/bp.2023.006},

doi = {10.32615/bp.2023.006},

issn = {00063134},

year  = {2023},

date = {2023-01-01},

urldate = {2023-01-01},

journal = {Biologia plantarum},

volume = {67},

number = {1},

pages = {67–74},

abstract = {Garlic mustard (Alliaria petiolata) is an herbaceous biennial plant native to Europe. In Ukraine, in addition to becoming a serious invader, garlic mustard can serve as a host to several viruses, which may affect agricultural crops. In view of this, the purpose of the study was to identify the virome of garlic mustard growing in Ukraine. Plant samples collected in Kyiv regions were tested for the presence of cucumber mosaic virus (CMV), turnip mosaic virus (TuMV), turnip yellow mosaic virus (TYMV), watermelon mosaic virus II (WMV), and turnip crinkle virus (TCV) by serological and/or molecular methods. According to the results found in the present study, symptomatic A. petiolata obtained in 2021 were infected with CMV (60%), TuMV (20%), or co-infected with CMV + TuMV (20%). TYMV, WMV II, and TCV were not detected in any of the collected samples. The cDNA fragments encoded the coat protein (CP) gene of CMV and TuMV were sequenced and named as CMV-Ap and TuMV-Ap, respectively. In phylogenetic analysis, the CMV-Ap closely resembled the German isolate MW582807 (Sarracenia sp.), with 99.8% nucleotide identity and belongs to subgroup II of CMV. In the phylogenetic tree, TuMV-Ap clustered with isolates AP017803, AP017764, AP017791, and JQ073722, and represented the highest identity (98.6%) to Iranian isolate IRNTRa9 (AP017803) from Rapistrum rugosum and Turkish isolate TUR49 (AP017872) from Raphanus raphanistrum. The sequences of CMV-Ap and TuMV-Ap were deposited in the GenBank under Accession Numbers MZ540213 and OM799323, respectively. The results obtained in the study indicate the important role of infected garlic mustard as alternative host and natural reservoir of CMV and TuMV from which these economically important viruses can spread to other wild and cultivated plants. This is the first molecular evidence of TuMV infection in A. petiolata from Ukraine.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{bpl-202301-0019,

title = {Thiosulphonate-rhamnolipid-glycanic complexes as inducers of virus resistance in hypersensitive plants},

author = {O Kovalenko and A Kyrychenko and V Lubenets and T Pokynbroda and Banya А and V Chervetsova and O Karpenko},

url = {https://doi.org/10.32615/bp.2023.014},

doi = {10.32615/bp.2023.014},

issn = {00063134},

year  = {2023},

date = {2023-01-01},

urldate = {2023-01-01},

journal = {Biologia plantarum},

volume = {67},

number = {1},

pages = {159–165},

abstract = {Involving the natural host-resistance mechanisms to pathogens are essential and one of the most promising approaches in development of first-line defenses against viral plant diseases. Polysaccharides isolated from natural sources are considered the most active resistance inducers. The biological activity of polysaccharides depends on the nature and chemical structure of the constituent components of complex preparations. In this view, the objective of our study was to evaluate the biological activity of complex preparations composed of glycans, rhamnolipids, and thiosulfonates as inducers of natural plant resistance and inhibitors of tobacco mosaic virus (TMV). Complex preparations were obtained using the following components: biogenic glycolipids - rhamnolipids of the Pseudomonas sp. strain PS-17, glycans - Ganoderma adspersum glucan and Candida maltosa mannan, as well as synthetic biocides - thiosulfonates (methylthiosulfanilate). The biological activity of the preparations was investigated in the host-virus model system Nicotiana tabacum L. and TMV. It was shown that preparations at concentrations of 10 and 100 μg mL^{-1} were active plant resistance inducers in N. tabacum cv. Immune 580, hypersensitive to TMV. At the same concentrations, complex preparations also reduced infectivity of TMV on Datura metel L. acting as viral infection inhibitors. The inducing activity of the complex preparations is sensitive to well-known transcription inhibitor actinomycin D (10 μg mL^{-1}). This fact may indicate the important role of RNA synthesis in the activation of plant virus resistance by the studied preparations.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kyrychenko_first_2022,

title = {First report of barley yellow dwarf virus-MAV infecting Avena sativa L. in Ukraine},

author = {Anhelina Kyrychenko and Halyna Snihur and Mykhailo Bohdan and Irena Budzanivska},

url = {https://link.springer.com/10.1007/s42161-022-01209-9},

doi = {10.1007/s42161-022-01209-9},

issn = {2239-7264},

year  = {2022},

date = {2022-08-01},

urldate = {2022-08-01},

journal = {Journal of Plant Pathology},

volume = {104},

number = {4},

pages = {1583–1583},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dunich_first_2022,

title = {First report of сucumber mosaic virus infecting Arctium lappa in Ukraine},

author = {Alina Dunich and Lidiya Mishchenko and Roksolana Sovinska and Anna Dashchenko and Anatoliy Mishchenko and Angelina Kyrychenko},

url = {https://link.springer.com/10.1007/s42161-022-01212-0},

doi = {10.1007/s42161-022-01212-0},

issn = {2239-7264},

year  = {2022},

date = {2022-08-01},

urldate = {2022-08-01},

journal = {Journal of Plant Pathology},

volume = {104},

number = {4},

pages = {1587–1588},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zabolotny_institute_of_microbiology_and_virology_nas_of_ukraine_survey_2022,

title = {A Survey of Grapevine Leafroll-Associated Virus 1 and 3 in the South of Ukraine and Development of Primers for GLRaV-3 Identification},

author = {AM Kyrychenko and KV Hrynchuk and IO Antipov and AI Konup},

url = {https://microbiolj.org.ua/en/archiv/2022-tom-84/3-may-jun-tom-84/2022-84-3-09/},

doi = {10.15407/microbiolj84.03.082},

issn = {10280987, 26169258},

year  = {2022},

date = {2022-05-02},

urldate = {2022-05-02},

journal = {Mikrobiolohichnyi Zhurnal},

volume = {84},

number = {3},

pages = {82–91},

abstract = {Viticulture is one of the most intensive and complex branches of Ukraine agriculture. Grapevine virus diseases are responsible for considerable economic losses to grape productivity and wine industries. One of the most notable and widespread viruses associated with vine leafroll disease is grapevine leafroll-associated viruses (GLRaV), belonging to the genus Ampelovirus, family Closteroviridae. Thus, the aim of this study was to conduct a survey targeting two viruses involved in the grapevine leafroll, namely grapevine leafroll-associated virus 1 (GLRaV-1) and virus 3(GLRaV-3) distributed in commercial wine grapes growing in the vineyards of the Ovidiopol and Bolhrad districts of the Odesa region. For efficient and accurate virus detection, we aimed to design universal primers based on conserved nucleotide sequences. Methods. Virus surveys of vineyards, visual diagnosis, immunoassay (ELISA), polymerase chain reaction with reverse transcription (RT-PCR), and Sanger sequencing of partial genome sequences of GLRaV. Results. The results obtained indicate that grapevine leafroll disease symptoms in field-grown grapevines in the south of Ukraine are caused by GLRaV-3. GLRaV-1 was not detected in any of the samples tested. To confirm the presence of GLRaV-3 in the samples, specific primers were designed targeting the coat protein region (GLRaV3-7f 5’-AGTAGGGGATGCAGCACAAG-3’; GLRaV3-7r 5’-ATCCAAAGCTATTCCCTTGC-3’) of the virus. A new set of primers (GLRaV3-7f / GLRaV3-7r) has been validated for sensitive detection of GLRaV-3 by RT-PCR and may be useful for routine virus detection in the laboratory as well as for large-scale testing. The partial coat protein gene of the isolate, GLRaV-3 ukr, distributed in the south of Ukraine, was sequenced, and the obtained sequence was deposited in GenBank under Acc. No. ON015835. The phylogenetic study demonstrated that GLRaV-3 ukr was closely related to isolates from Russia (MZ065372 and MZ065370).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kyrychenko_first_2022b,

title = {First report of cucumber mosaic virus infecting Alliaria petiolata in Ukraine},

author = {Angelina Kyrychenko and Halyna Snihur and Tetiana Shevchenko},

url = {https://link.springer.com/10.1007/s42161-022-01107-0},

doi = {10.1007/s42161-022-01107-0},

issn = {2239-7264},

year  = {2022},

date = {2022-04-01},

urldate = {2022-04-01},

journal = {Journal of Plant Pathology},

volume = {104},

number = {3},

pages = {1141–1141},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zabolotny_institute_of_microbiology_and_virology_nas_of_ukraine_first_2021,

title = {First Report of Potato Viruses Infecting Lamium purpureum in Ukraine},

author = {AM. Kyrychenko and MM Bohdan and HO.Snihur and IS Shcherbatenko IO Antipov},

url = {http://microbiolj.org.ua/en/archiv/2021-tom-83/6-nov-dec-tom-83/2021-83-6-07/},

doi = {10.15407/microbiolj83.06.055},

issn = {10280987, 26169258},

year  = {2021},

date = {2021-12-01},

urldate = {2021-12-01},

journal = {Mikrobiolohichnyi Zhurnal},

volume = {83},

number = {6},

pages = {55–64},

abstract = {Weeds as reservoirs for destructive plant pathogens have a significant impact on the viral epidemiology, ecology and, as a result, on local economy, and are therefore being investigated in many parts of the world. Thus, the aim of this study was to investigate virus occurrence in red dead-nettle plants (Lamium purpureum L.) widespread in urban and field conditions throughout the in the Kyiv region of Ukraine. Methods. Field crop observations, visual diagnosis, biological testing of the virus, immunoassay (ELISA), polymerase chain reaction with reverse transcription (RT-PCR), sanger sequencing of partial genome sequences of PVX, PVY, PVS, PVM. Results. The results obtained in the study indicate that Lamium plants could be alternative weed hosts of number important viral diseases including potatoes and other vegetables. Serological and molecular test results evidence plants were infected by Potato virus X, Potato virus Y, Potato virus M, Potato virus S and therefore Lamium L. species can serve as a potential source of inoculum for wide range of vegetables and ornamentals. This study is the first report of Lamium plants being naturally infected with Potato virus M and Potato virus S in central Europe. Conclusions. These plants are alternative host of mixed infection with viruses belonging to different families: Alphaflexiviridae, Betaflexiviridae and Potyviridae.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{kyrychenko_viruses_2021,

title = {Viruses of Wild Plants and Current Metagenomic Methods for Their Investigation},

author = {AN Kyrychenko and IS Shcherbatenko and AG Kovalenko},

url = {https://link.springer.com/10.3103/S0095452721030038},

doi = {10.3103/S0095452721030038},

issn = {0095-4527, 1934-9440},

year  = {2021},

date = {2021-05-01},

urldate = {2021-05-01},

journal = {Cytology and Genetics},

volume = {55},

number = {3},

pages = {248–255},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zabolotny_institute_of_microbiology_and_virology_nas_of_ukraine_anti-tmv_2021,

title = {Anti-TMV Activities of Pantoea agglomerans Lipopolysaccharides in vitro},

author = {TV Bulyhina and AM Kyrychenko and MS Kharchuk and LD Varbanets},

url = {http://microbiolj.org.ua/en/archiv/2021-tom-83/2-mar-apr-tom-83/2021-83-2-07/},

doi = {10.15407/microbiolj83.02.064},

issn = {10280987, 26169258},

year  = {2021},

date = {2021-04-01},

urldate = {2021-04-01},

journal = {Mikrobiolohichnyi Zhurnal},

volume = {83},

number = {2},

pages = {64–72},

abstract = {Today there are no antiviral drugs of chemical nature that can completely cure virus-infected plants. The fact that their effect is limited to minimizing the pathogenic effect of viruses motivates many researchers to look for alternatives. In recent years it has been shown that lipopolysaccharides (LPS) of some bacteria, in particular representatives of the Pseudomonas genus were active against Tobacco mosaic virus (TMV). Therefore, we were interested in the additional study of LPS of phytopathogenic bacteria Pantoea agglomerans as a possible drug acting as antiviral agent. The aim of current study was to evaluate the antiviral activities of LPS obtained from phytopathogenic bacteria P. agglomerans against TMV in vitro. Methods. The antiviral activity of LPS preparations was investigated in vitro and assessed according to the inhibition percentage towards the number of local lesions in Datura stramonium leaves. P. agglomerans LPS was isolated from dry bacterial mass by phenol-water method. LPS mild acid degradation allowed to separate O-specific polysaccharide (OPS) and lipid A, which structures were identified by us earlier. The analysis of TMV and LPS interactions was carried out using a JEM 1400 transmission electron microscope (Jeol, Japan) at an accelerating voltage of 80 kV. Results. The most active were LPS preparations from P. agglomerans P324 and 8488. In vitro inhibitory efficacies of TMV infection by these LPS preparations was 59 and 60% respectively. LPS preparations of P. agglomerans 7969, 7604 and 9637, on the contrary, were inactive. Comparative analysis of the antiviral activity of LPS structural components of two P. agglomerans P324 and 7604 strains showed that the greatest inhibitory effect on the infectivity of TMV was exhibited by P. agglomerans P324 lipid A, the antiviral activity of which practically did not differ from the activity of the LPS molecule (it was lower by 7%). At the same time, the inhibitory effect of P. agglomerans 7604 core oligosaccharide (OG-core) against TMV was slightly higher compared to the effect of the whole LPS molecule. It can be assumed that the OG-core stimulated the defense mechanisms of plants and prevented the development of viral infection. Electron microscopic dates have shown that P. agglomerans P324 LPS at the concentration of 1 mg/ml influenced on freely located virions in the control causing “sticking” thus forming dense clusters, complexes or “bundles” of the virus. The individual structural components of P. agglomerans P324 LPS (lipid A and OG-core) did not have the same effect as a whole molecule. Conclusions. The study of the antiviral activity of LPS in the model system TMV – Datura stramonium L. plants showed that the most active were LPS preparations of only two strains of P. agglomerans (P324 and 8488) while the other seven strains were inactive. Individual structural components: lipid A from P. agglomerans P324 and OG-core from P. agglomerans 7604 decreased the infectivity of TMV by 7 and 15% higher than the initial LPS molecule. According to electron microscopy data the virions sticked together forming the dense clusters in case of the direct LPS-virus contacting in vitro whereas in the control it was observed just a single free virus particles. A more detailed study of the effect of individual structural components will help to understand the regularities of the LPS structure effect on TMV infectivity.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zabolotny_institute_of_microbiology_and_virology_nas_of_ukraine_weeds_2020,

title = {Weeds as Reservoirs of Viruses in Agrobiocenoses of Legumes in Ukraine},

author = {AN Kyrychenko and MM Bohdan and IS Shcherbatenko},

url = {http://microbiolj.org.ua/en/archiv/2020-tom-82/6-nov-dec-tom-82/2020-82-6-10/},

doi = {10.15407/microbiolj82.06.094},

issn = {10280987, 26169258},

year  = {2020},

date = {2020-11-01},

urldate = {2020-11-01},

journal = {Mikrobiolohichnyi Zhurnal},

volume = {82},

number = {6},

pages = {94–106},

abstract = {This paper is the review of literature data on the prevalence of weeds as possible reservoirs of plant viruses in agroecosystems of Ukraine. The information presented here about the most distributed weeds as reservoirs of harmful plant viruses will be useful for understanding the pathogens ecology, analyzing the virus epidemiology and for disease management. Since legumes are the main crops grown in Ukraine, the paper focuses on weeds spreading in the agrosystems of cultivated plant. The paper provides information about the primary sources of soybean viruses (Soybean mosaic virus, Cucumber mosaic virus, Alfalfa mosaic virus, Tomato ringspot virus) and bean viruses (Bean yellow mosaic virus, Bean common mosaic virus) as well as the main factors contributing the virus transmission in agrocenosis.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zabolotny_institute_of_microbiology_and_virology_nas_of_ukraine_virus_2020,

title = {Virus Infected Bean Tissue Culture Cells and It’s Healing in vitro Using Liposomal form of Glycanes},

author = {OH Kovalenko and A Kyrychenko and OY Kovalenko},

url = {http://microbiolj.org.ua/en/archiv/2020-tom-82/5-sep-oct-tom-82/2020-82-5-08/},

doi = {10.15407/microbiolj82.05.058},

issn = {10280987, 26169258},

year  = {2020},

date = {2020-10-01},

urldate = {2020-10-01},

journal = {Mikrobiolohichnyi Zhurnal},

volume = {82},

number = {5},

pages = {58–64},

abstract = {The aim of the study was to develop a recovery means for beans infected by Bean yellow mosaic virus (BYMV) as well as Bean common mosaic virus (BCMV) using callus culture and liposomal glycan preparations. Methods. Cultivation of explants and callus cultures was carried out in vitro using conventional methods of plant biotechnology. The tissue culture propagation was performed during the spring or summer seasons. The presence of viral infection was tested by reverse transcription polymerase chain reaction. The virus-specific primers that allowed amplifying the conserved regions of the capsid protein gene of BCMV or BYMV were used for virus identification. Results. The culture of bean callus infected with BCMV was obtained and adapted for antiviral agents testing. It has been shown that during long-term cultivation (10–12 weeks) in the presence of liposomal preparation containing Ganoderma adspersum glucan (10–100 mg/l), plant tissue culture become free from viruses following virus eradication. This is evidenced by the absence in the callus tissue of 391 bp sequences typical for the virus coat protein gene. Conclusions. The full suppression of virus reproduction and gradual elimination of virus occurred in callus tissue obtained from BCMV-infected beans and cultured on B-5 medium supplemented with liposomal glycanglycolipid complex (10–100 mg/l). The data obtained can be useful for the development of practical control method to cure plant virus diseases using callus culture and antiviral-active glycan-glycolipid complexes.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zabolotny_institute_of_microbiology_and_virology_nas_of_ukraine_physical_2020,

title = {The Physical Properties of Bean Common Mosaic Virus Distributed in Ukraine},

author = {A Kyrychenko and V Prylipko},

url = {http://microbiolj.org.ua/en/archiv/2020-tom-82/3-may-jun-tom-82/2020-82-3-07/},

doi = {10.15407/microbiolj82.03.065},

issn = {10280987, 26169258},

year  = {2020},

date = {2020-06-01},

urldate = {2020-06-01},

journal = {Mikrobiolohichnyi Zhurnal},

volume = {82},

number = {3},

pages = {65–70},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{national_university_of_life_and_environmental_sciences_of_ukraine_seed_2020,

title = {Seed transmission of bean common mosaic virus in phaseolus vulgaris cv Chervona Shapochka},

author = {A Kyrychenko and I Antipov and K Hrynchuk and A Likhanov},

url = {http://journals.nubip.edu.ua/index.php/Biologiya/article/view/13993},

doi = {10.31548/biologiya2020.01.069},

issn = {27068382, 27068390},

year  = {2020},

date = {2020-05-01},

urldate = {2020-05-01},

journal = {Biological Systems: Theory and Innovation},

volume = {11},

number = {1},

pages = {69–79},

keywords = {},

pubstate = {published},

tppubtype = {article}

}