dr Daria Tashyreva
Scientific interests
- Taxonomy, ecology, physiology, diversity, and ultrastructure of euglenozoan protists.
- Diversity and molecular interactions of bacterial and archaeal symbionts with protists.
Ongoing projects
- Analysis of functional and taxonomic diversity of symbionts among euglenozoan protists (SYMBIOZOANS). Read more here
Aims of the project:
- Investigating currently overlooked frequency and diversity of prokaryotic endosymbionts among cultured representatives of euglenozoan protists.
- Performing morphological, taxonomic, and functional analysis of selected cultured euglenozoans in order to identify the nature of host-symbiont relationships and investigate the role of each of the partners.
- Evaluating the frequency and diversity of endosymbiotic relationships in natural communities of euglenozoans from freshwater and marine environments.
- Taxonomy and ultrastructure of diplonemid protists and their molecular interaction with bacterial symbionts, in collaboration with the Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.
Publications
2023
Tashyreva, Daria; Týč, Jiří; Horák, Aleš; Lukeš, Julius
Ultrastructure and 3D reconstruction of a diplonemid protist (Diplonemea) and its novel membranous organelle Journal Article
In: mBio, vol. 14, no. 5, 2023, ISSN: 2150-7511.
@article{Tashyreva2023,
title = {Ultrastructure and 3D reconstruction of a diplonemid protist (Diplonemea) and its novel membranous organelle},
author = {Daria Tashyreva and Jiří Týč and Aleš Horák and Julius Lukeš},
editor = {Joseph Heitman},
doi = {10.1128/mbio.01921-23},
issn = {2150-7511},
year = {2023},
date = {2023-10-31},
urldate = {2023-10-31},
journal = {mBio},
volume = {14},
number = {5},
publisher = {American Society for Microbiology},
abstract = {<jats:title>ABSTRACT</jats:title>
<jats:p>
Although diplonemid protists (Diplonemea, Euglenozoa) are among the most species-rich microeukaryotes in the ocean, many ultrastructural features of this lineage remain to be clarified. Using serial block-face scanning and transmission electron microscopy, we provide the whole-cell three-dimensional model of a previously undescribed diplonemid
<jats:italic>Lacrimia vacuolata</jats:italic>
sp. nov. For the first time, we were able to reconstruct a diplonemid cell in detail, including all its organelles and the flagellar and feeding apparatuses. By analyzing various stages of the cell cycle, we provide the first description of a diplonemid cell division, which is characterized by the disassembly of flagellar axonemes and the feeding apparatus and their further construction in the daughter cells. Moreover, we have identified a novel, ultrastructurally complex organelle, herein named the colv (
<jats:underline>C</jats:underline>
enter for
<jats:underline>O</jats:underline>
rganization of
<jats:underline>L</jats:underline>
ayered
<jats:underline>V</jats:underline>
esicles). We suggest that the colv is involved in food processing and membrane trafficking, and describe its close association with other components of the cellular digestive system.
</jats:p>
<jats:sec>
<jats:title>IMPORTANCE</jats:title>
<jats:p>The knowledge of cell biology of a eukaryotic group is essential for correct interpretation of ecological and molecular data. Although diplonemid protists are one of the most species-rich lineages of marine eukaryotes, only very fragmentary information is available about the cellular architecture of this taxonomically diverse group. Here, a large serial block-face scanning electron microscopy data set complemented with light and fluorescence microscopy allowed the first detailed three-dimensional reconstruction of a diplonemid species. We describe numerous previously unknown peculiarities of the cellular architecture and cell division characteristic for diplonemid flagellates, and illustrate the obtained results with multiple three-dimensional models, comprehensible for non-specialists in protist ultrastructure.</jats:p>
</jats:sec>},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
<jats:title>ABSTRACT</jats:title>
<jats:p>
Although diplonemid protists (Diplonemea, Euglenozoa) are among the most species-rich microeukaryotes in the ocean, many ultrastructural features of this lineage remain to be clarified. Using serial block-face scanning and transmission electron microscopy, we provide the whole-cell three-dimensional model of a previously undescribed diplonemid
<jats:italic>Lacrimia vacuolata</jats:italic>
sp. nov. For the first time, we were able to reconstruct a diplonemid cell in detail, including all its organelles and the flagellar and feeding apparatuses. By analyzing various stages of the cell cycle, we provide the first description of a diplonemid cell division, which is characterized by the disassembly of flagellar axonemes and the feeding apparatus and their further construction in the daughter cells. Moreover, we have identified a novel, ultrastructurally complex organelle, herein named the colv (
<jats:underline>C</jats:underline>
enter for
<jats:underline>O</jats:underline>
rganization of
<jats:underline>L</jats:underline>
ayered
<jats:underline>V</jats:underline>
esicles). We suggest that the colv is involved in food processing and membrane trafficking, and describe its close association with other components of the cellular digestive system.
</jats:p>
<jats:sec>
<jats:title>IMPORTANCE</jats:title>
<jats:p>The knowledge of cell biology of a eukaryotic group is essential for correct interpretation of ecological and molecular data. Although diplonemid protists are one of the most species-rich lineages of marine eukaryotes, only very fragmentary information is available about the cellular architecture of this taxonomically diverse group. Here, a large serial block-face scanning electron microscopy data set complemented with light and fluorescence microscopy allowed the first detailed three-dimensional reconstruction of a diplonemid species. We describe numerous previously unknown peculiarities of the cellular architecture and cell division characteristic for diplonemid flagellates, and illustrate the obtained results with multiple three-dimensional models, comprehensible for non-specialists in protist ultrastructure.</jats:p>
</jats:sec>
<jats:p>
Although diplonemid protists (Diplonemea, Euglenozoa) are among the most species-rich microeukaryotes in the ocean, many ultrastructural features of this lineage remain to be clarified. Using serial block-face scanning and transmission electron microscopy, we provide the whole-cell three-dimensional model of a previously undescribed diplonemid
<jats:italic>Lacrimia vacuolata</jats:italic>
sp. nov. For the first time, we were able to reconstruct a diplonemid cell in detail, including all its organelles and the flagellar and feeding apparatuses. By analyzing various stages of the cell cycle, we provide the first description of a diplonemid cell division, which is characterized by the disassembly of flagellar axonemes and the feeding apparatus and their further construction in the daughter cells. Moreover, we have identified a novel, ultrastructurally complex organelle, herein named the colv (
<jats:underline>C</jats:underline>
enter for
<jats:underline>O</jats:underline>
rganization of
<jats:underline>L</jats:underline>
ayered
<jats:underline>V</jats:underline>
esicles). We suggest that the colv is involved in food processing and membrane trafficking, and describe its close association with other components of the cellular digestive system.
</jats:p>
<jats:sec>
<jats:title>IMPORTANCE</jats:title>
<jats:p>The knowledge of cell biology of a eukaryotic group is essential for correct interpretation of ecological and molecular data. Although diplonemid protists are one of the most species-rich lineages of marine eukaryotes, only very fragmentary information is available about the cellular architecture of this taxonomically diverse group. Here, a large serial block-face scanning electron microscopy data set complemented with light and fluorescence microscopy allowed the first detailed three-dimensional reconstruction of a diplonemid species. We describe numerous previously unknown peculiarities of the cellular architecture and cell division characteristic for diplonemid flagellates, and illustrate the obtained results with multiple three-dimensional models, comprehensible for non-specialists in protist ultrastructure.</jats:p>
</jats:sec>
Zakharova, Alexandra; Tashyreva, Daria; Butenko, Anzhelika; Morales, Jorge; Saura, Andreu; Svobodová, Michaela; Poschmann, Gereon; Nandipati, Satish; Zakharova, Alena; Noyvert, David; Gahura, Ondřej; Týč, Jiří; Stühler, Kai; Kostygov, Alexei Y.; Nowack, Eva C. M.; Lukeš, Julius; Yurchenko, Vyacheslav
In: Current Biology, vol. 33, no. 13, pp. 2690–2701.e5, 2023, ISSN: 0960-9822.
@article{Zakharova2023,
title = {A neo-functionalized homolog of host transmembrane protein controls localization of bacterial endosymbionts in the trypanosomatid Novymonas esmeraldas},
author = {Alexandra Zakharova and Daria Tashyreva and Anzhelika Butenko and Jorge Morales and Andreu Saura and Michaela Svobodová and Gereon Poschmann and Satish Nandipati and Alena Zakharova and David Noyvert and Ondřej Gahura and Jiří Týč and Kai Stühler and Alexei Y. Kostygov and Eva C.M. Nowack and Julius Lukeš and Vyacheslav Yurchenko},
doi = {10.1016/j.cub.2023.04.060},
issn = {0960-9822},
year = {2023},
date = {2023-07-00},
urldate = {2023-07-00},
journal = {Current Biology},
volume = {33},
number = {13},
pages = {2690--2701.e5},
publisher = {Elsevier BV},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
George, Emma E.; Barcytė, Dovilė; Lax, Gordon; Livingston, Sam; Tashyreva, Daria; Husnik, Filip; Lukeš, Julius; Eliáš, Marek; Keeling, Patrick J.
A single cryptomonad cell harbors a complex community of organelles, bacteria, a phage, and selfish elements Journal Article
In: Current Biology, vol. 33, no. 10, pp. 1982–1996.e4, 2023, ISSN: 0960-9822.
@article{George2023,
title = {A single cryptomonad cell harbors a complex community of organelles, bacteria, a phage, and selfish elements},
author = {Emma E. George and Dovilė Barcytė and Gordon Lax and Sam Livingston and Daria Tashyreva and Filip Husnik and Julius Lukeš and Marek Eliáš and Patrick J. Keeling},
doi = {10.1016/j.cub.2023.04.010},
issn = {0960-9822},
year = {2023},
date = {2023-05-00},
urldate = {2023-05-00},
journal = {Current Biology},
volume = {33},
number = {10},
pages = {1982--1996.e4},
publisher = {Elsevier BV},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Pilátová, Jana; Tashyreva, Daria; Týč, Jiří; Vancová, Marie; Bokhari, Syed Nadeem Hussain; Skoupý, Radim; Klementová, Mariana; Küpper, Hendrik; Mojzeš, Peter; Lukeš, Julius
Massive Accumulation of Strontium and Barium in Diplonemid Protists Journal Article
In: mBio, vol. 14, no. 1, 2023, ISSN: 2150-7511.
@article{Pilátová2023,
title = {Massive Accumulation of Strontium and Barium in Diplonemid Protists},
author = {Jana Pilátová and Daria Tashyreva and Jiří Týč and Marie Vancová and Syed Nadeem Hussain Bokhari and Radim Skoupý and Mariana Klementová and Hendrik Küpper and Peter Mojzeš and Julius Lukeš},
editor = {L. David Sibley},
doi = {10.1128/mbio.03279-22},
issn = {2150-7511},
year = {2023},
date = {2023-02-28},
urldate = {2023-02-28},
journal = {mBio},
volume = {14},
number = {1},
publisher = {American Society for Microbiology},
abstract = {<jats:p>
We have identified that diplonemids, an abundant group of marine planktonic protists, accumulate conspicuous amounts of Sr
<jats:sup>2+</jats:sup>
and Ba
<jats:sup>2+</jats:sup>
in the form of intracellular barite and celestite crystals, in concentrations that greatly exceed those of the most efficient Ba/Sr-accumulating organisms known to date. We propose that diplonemids are potential players in Ba
<jats:sup>2+</jats:sup>
/Sr
<jats:sup>2+</jats:sup>
cycling in the ocean and have possibly contributed to sedimentary rock formation over long geological periods.
</jats:p>},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
<jats:p>
We have identified that diplonemids, an abundant group of marine planktonic protists, accumulate conspicuous amounts of Sr
<jats:sup>2+</jats:sup>
and Ba
<jats:sup>2+</jats:sup>
in the form of intracellular barite and celestite crystals, in concentrations that greatly exceed those of the most efficient Ba/Sr-accumulating organisms known to date. We propose that diplonemids are potential players in Ba
<jats:sup>2+</jats:sup>
/Sr
<jats:sup>2+</jats:sup>
cycling in the ocean and have possibly contributed to sedimentary rock formation over long geological periods.
</jats:p>
We have identified that diplonemids, an abundant group of marine planktonic protists, accumulate conspicuous amounts of Sr
<jats:sup>2+</jats:sup>
and Ba
<jats:sup>2+</jats:sup>
in the form of intracellular barite and celestite crystals, in concentrations that greatly exceed those of the most efficient Ba/Sr-accumulating organisms known to date. We propose that diplonemids are potential players in Ba
<jats:sup>2+</jats:sup>
/Sr
<jats:sup>2+</jats:sup>
cycling in the ocean and have possibly contributed to sedimentary rock formation over long geological periods.
</jats:p>
2022
George, Emma E; Tashyreva, Daria; Kwong, Waldan K; Okamoto, Noriko; Horák, Aleš; Husnik, Filip; Lukeš, Julius; Keeling, Patrick J
Gene Transfer Agents in Bacterial Endosymbionts of Microbial Eukaryotes Journal Article
In: vol. 14, no. 7, 2022, ISSN: 1759-6653.
@article{George2022,
title = {Gene Transfer Agents in Bacterial Endosymbionts of Microbial Eukaryotes},
author = {Emma E George and Daria Tashyreva and Waldan K Kwong and Noriko Okamoto and Aleš Horák and Filip Husnik and Julius Lukeš and Patrick J Keeling},
editor = {Tal Dagan},
doi = {10.1093/gbe/evac099},
issn = {1759-6653},
year = {2022},
date = {2022-07-02},
urldate = {2022-07-02},
volume = {14},
number = {7},
publisher = {Oxford University Press (OUP)},
abstract = {<jats:title>Abstract</jats:title>
<jats:p>Gene transfer agents (GTAs) are virus-like structures that package and transfer prokaryotic DNA from donor to recipient prokaryotic cells. Here, we describe widespread GTA gene clusters in the highly reduced genomes of bacterial endosymbionts from microbial eukaryotes (protists). Homologs of the GTA capsid and portal complexes were initially found to be present in several highly reduced alphaproteobacterial endosymbionts of diplonemid protists (Rickettsiales and Rhodospirillales). Evidence of GTA expression was found in polyA-enriched metatranscriptomes of the diplonemid hosts and their endosymbionts, but due to biases in the polyA-enrichment methods, levels of GTA expression could not be determined. Examining the genomes of closely related bacteria revealed that the pattern of retained GTA head/capsid complexes with missing tail components was common across Rickettsiales and Holosporaceae (Rhodospirillales), all obligate symbionts with a wide variety of eukaryotic hosts. A dN/dS analysis of Rickettsiales and Holosporaceae symbionts revealed that purifying selection is likely the main driver of GTA evolution in symbionts, suggesting they remain functional, but the ecological function of GTAs in bacterial symbionts is unknown. In particular, it is unclear how increasing horizontal gene transfer in small, largely clonal endosymbiont populations can explain GTA retention, and, therefore, the structures may have been repurposed in endosymbionts for host interactions. Either way, their widespread retention and conservation in endosymbionts of diverse eukaryotes suggests an important role in symbiosis.</jats:p>},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
<jats:title>Abstract</jats:title>
<jats:p>Gene transfer agents (GTAs) are virus-like structures that package and transfer prokaryotic DNA from donor to recipient prokaryotic cells. Here, we describe widespread GTA gene clusters in the highly reduced genomes of bacterial endosymbionts from microbial eukaryotes (protists). Homologs of the GTA capsid and portal complexes were initially found to be present in several highly reduced alphaproteobacterial endosymbionts of diplonemid protists (Rickettsiales and Rhodospirillales). Evidence of GTA expression was found in polyA-enriched metatranscriptomes of the diplonemid hosts and their endosymbionts, but due to biases in the polyA-enrichment methods, levels of GTA expression could not be determined. Examining the genomes of closely related bacteria revealed that the pattern of retained GTA head/capsid complexes with missing tail components was common across Rickettsiales and Holosporaceae (Rhodospirillales), all obligate symbionts with a wide variety of eukaryotic hosts. A dN/dS analysis of Rickettsiales and Holosporaceae symbionts revealed that purifying selection is likely the main driver of GTA evolution in symbionts, suggesting they remain functional, but the ecological function of GTAs in bacterial symbionts is unknown. In particular, it is unclear how increasing horizontal gene transfer in small, largely clonal endosymbiont populations can explain GTA retention, and, therefore, the structures may have been repurposed in endosymbionts for host interactions. Either way, their widespread retention and conservation in endosymbionts of diverse eukaryotes suggests an important role in symbiosis.</jats:p>
<jats:p>Gene transfer agents (GTAs) are virus-like structures that package and transfer prokaryotic DNA from donor to recipient prokaryotic cells. Here, we describe widespread GTA gene clusters in the highly reduced genomes of bacterial endosymbionts from microbial eukaryotes (protists). Homologs of the GTA capsid and portal complexes were initially found to be present in several highly reduced alphaproteobacterial endosymbionts of diplonemid protists (Rickettsiales and Rhodospirillales). Evidence of GTA expression was found in polyA-enriched metatranscriptomes of the diplonemid hosts and their endosymbionts, but due to biases in the polyA-enrichment methods, levels of GTA expression could not be determined. Examining the genomes of closely related bacteria revealed that the pattern of retained GTA head/capsid complexes with missing tail components was common across Rickettsiales and Holosporaceae (Rhodospirillales), all obligate symbionts with a wide variety of eukaryotic hosts. A dN/dS analysis of Rickettsiales and Holosporaceae symbionts revealed that purifying selection is likely the main driver of GTA evolution in symbionts, suggesting they remain functional, but the ecological function of GTAs in bacterial symbionts is unknown. In particular, it is unclear how increasing horizontal gene transfer in small, largely clonal endosymbiont populations can explain GTA retention, and, therefore, the structures may have been repurposed in endosymbionts for host interactions. Either way, their widespread retention and conservation in endosymbionts of diverse eukaryotes suggests an important role in symbiosis.</jats:p>
Tashyreva, Daria; Simpson, Alastair G. B.; Prokopchuk, Galina; Škodová-Sveráková, Ingrid; Butenko, Anzhelika; Hammond, Michael; George, Emma E.; Flegontova, Olga; Záhonová, Kristína; Faktorová, Drahomíra; Yabuki, Akinori; Horák, Aleš; Keeling, Patrick J.; Lukeš, Julius
Diplonemids – A Review on "new" flagellates on the oceanic block Journal Article
In: Protist, vol. 173, no. 2, 2022, ISSN: 1434-4610.
@article{Tashyreva2022,
title = {Diplonemids – A Review on "new" flagellates on the oceanic block},
author = {Daria Tashyreva and Alastair G.B. Simpson and Galina Prokopchuk and Ingrid Škodová-Sveráková and Anzhelika Butenko and Michael Hammond and Emma E. George and Olga Flegontova and Kristína Záhonová and Drahomíra Faktorová and Akinori Yabuki and Aleš Horák and Patrick J. Keeling and Julius Lukeš},
doi = {10.1016/j.protis.2022.125868},
issn = {1434-4610},
year = {2022},
date = {2022-04-00},
urldate = {2022-04-00},
journal = {Protist},
volume = {173},
number = {2},
publisher = {Elsevier BV},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2021
Husnik, Filip; Tashyreva, Daria; Boscaro, Vittorio; George, Emma E.; Lukeš, Julius; Keeling, Patrick J.
Bacterial and archaeal symbioses with protists Journal Article
In: Current Biology, vol. 31, no. 13, pp. R862–R877, 2021, ISSN: 0960-9822.
@article{Husnik2021,
title = {Bacterial and archaeal symbioses with protists},
author = {Filip Husnik and Daria Tashyreva and Vittorio Boscaro and Emma E. George and Julius Lukeš and Patrick J. Keeling},
doi = {10.1016/j.cub.2021.05.049},
issn = {0960-9822},
year = {2021},
date = {2021-07-00},
urldate = {2021-07-00},
journal = {Current Biology},
volume = {31},
number = {13},
pages = {R862--R877},
publisher = {Elsevier BV},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Kostygov, Alexei Y; Karnkowska, Anna; Votypka, Jan; Tashyreva, Daria; Maciszewski, Kacper; Yurchenko, Vyacheslav; Lukes, Julius
Euglenozoa : taxonomy, diversity and ecology, symbioses and viruses Journal Article
In: Open Biology, vol. 11, no. 3, pp. 200407-200407, 2021, ISBN: 0000000337.
@article{Kostygov2021,
title = {Euglenozoa : taxonomy, diversity and ecology, symbioses and viruses},
author = {Alexei Y Kostygov and Anna Karnkowska and Jan Votypka and Daria Tashyreva and Kacper Maciszewski and Vyacheslav Yurchenko and Julius Lukes},
url = {https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.200407},
doi = {https://doi.org/10.1098/rsob.200407},
isbn = {0000000337},
year = {2021},
date = {2021-01-01},
urldate = {2021-01-01},
journal = {Open Biology},
volume = {11},
number = {3},
pages = {200407-200407},
abstract = {Euglenozoa is a species-rich group of protists, which have extremely diverse
lifestyles and a range of features that distinguish them from other eukaryotes. They are composed of free-living and parasitic kinetoplastids,
mostly free-living diplonemids, heterotrophic and photosynthetic euglenids,
as well as deep-sea symbiontids. Although they form a well-supported
monophyletic group, these morphologically rather distinct groups are
almost never treated together in a comparative manner, as attempted here.
We present an updated taxonomy, complemented by photos of representative species, with notes on diversity, distribution and biology of
euglenozoans. For kinetoplastids, we propose a significantly modified taxonomy that reflects the latest findings. Finally, we summarize what is
known about viruses infecting euglenozoans, as well as their relationships
with ecto- and endosymbiotic bacteria.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Euglenozoa is a species-rich group of protists, which have extremely diverse
lifestyles and a range of features that distinguish them from other eukaryotes. They are composed of free-living and parasitic kinetoplastids,
mostly free-living diplonemids, heterotrophic and photosynthetic euglenids,
as well as deep-sea symbiontids. Although they form a well-supported
monophyletic group, these morphologically rather distinct groups are
almost never treated together in a comparative manner, as attempted here.
We present an updated taxonomy, complemented by photos of representative species, with notes on diversity, distribution and biology of
euglenozoans. For kinetoplastids, we propose a significantly modified taxonomy that reflects the latest findings. Finally, we summarize what is
known about viruses infecting euglenozoans, as well as their relationships
with ecto- and endosymbiotic bacteria.
lifestyles and a range of features that distinguish them from other eukaryotes. They are composed of free-living and parasitic kinetoplastids,
mostly free-living diplonemids, heterotrophic and photosynthetic euglenids,
as well as deep-sea symbiontids. Although they form a well-supported
monophyletic group, these morphologically rather distinct groups are
almost never treated together in a comparative manner, as attempted here.
We present an updated taxonomy, complemented by photos of representative species, with notes on diversity, distribution and biology of
euglenozoans. For kinetoplastids, we propose a significantly modified taxonomy that reflects the latest findings. Finally, we summarize what is
known about viruses infecting euglenozoans, as well as their relationships
with ecto- and endosymbiotic bacteria.
2020
Kostygov, Alexei Y.; Frolov, Alexander O.; Malysheva, Marina N.; Ganyukova, Anna I.; Chistyakova, Lyudmila V.; Tashyreva, Daria; Tesařová, Martina; Spodareva, Viktoria V.; Režnarová, Jana; Macedo, Diego H.; Butenko, Anzhelika; d’Avila-Levy, Claudia M.; Lukeš, Julius; Yurchenko, Vyacheslav
Vickermania gen. nov., trypanosomatids that use two joined flagella to resist midgut peristaltic flow within the fly host Journal Article
In: BMC Biol, vol. 18, no. 1, 2020, ISSN: 1741-7007.
@article{Kostygov2020,
title = {Vickermania gen. nov., trypanosomatids that use two joined flagella to resist midgut peristaltic flow within the fly host},
author = {Alexei Y. Kostygov and Alexander O. Frolov and Marina N. Malysheva and Anna I. Ganyukova and Lyudmila V. Chistyakova and Daria Tashyreva and Martina Tesařová and Viktoria V. Spodareva and Jana Režnarová and Diego H. Macedo and Anzhelika Butenko and Claudia M. d’Avila-Levy and Julius Lukeš and Vyacheslav Yurchenko},
doi = {10.1186/s12915-020-00916-y},
issn = {1741-7007},
year = {2020},
date = {2020-12-00},
urldate = {2020-12-00},
journal = {BMC Biol},
volume = {18},
number = {1},
publisher = {Springer Science and Business Media LLC},
abstract = {<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Background</jats:title>
<jats:p>The family Trypanosomatidae encompasses parasitic flagellates, some of which cause serious vector-transmitted diseases of humans and domestic animals. However, insect-restricted parasites represent the ancestral and most diverse group within the family. They display a range of unusual features and their study can provide insights into the biology of human pathogens. Here we describe <jats:italic>Vickermania</jats:italic>, a new genus of fly midgut-dwelling parasites that bear two flagella in contrast to other trypanosomatids, which are unambiguously uniflagellate.</jats:p>
</jats:sec><jats:sec>
<jats:title>Results</jats:title>
<jats:p><jats:italic>Vickermania</jats:italic> has an odd cell cycle, in which shortly after the division the uniflagellate cell starts growing a new flagellum attached to the old one and preserves their contact until the late cytokinesis. The flagella connect to each other throughout their whole length and carry a peculiar seizing structure with a paddle-like apex and two lateral extensions at their tip. In contrast to typical trypanosomatids, which attach to the insect host’s intestinal wall, <jats:italic>Vickermania</jats:italic> is separated from it by a continuous peritrophic membrane and resides freely in the fly midgut lumen.</jats:p>
</jats:sec><jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>We propose that <jats:italic>Vickermania</jats:italic> developed a survival strategy that relies on constant movement preventing discharge from the host gut due to intestinal peristalsis. Since these parasites cannot attach to the midgut wall, they were forced to shorten the period of impaired motility when two separate flagella in dividing cells interfere with each other. The connection between the flagella ensures their coordinate movement until the separation of the daughter cells. We propose that <jats:italic>Trypanosoma brucei</jats:italic>, a severe human pathogen, during its development in the tsetse fly midgut faces the same conditions and follows the same strategy as <jats:italic>Vickermania</jats:italic> by employing an analogous adaptation, the flagellar connector.</jats:p>
</jats:sec>},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Background</jats:title>
<jats:p>The family Trypanosomatidae encompasses parasitic flagellates, some of which cause serious vector-transmitted diseases of humans and domestic animals. However, insect-restricted parasites represent the ancestral and most diverse group within the family. They display a range of unusual features and their study can provide insights into the biology of human pathogens. Here we describe <jats:italic>Vickermania</jats:italic>, a new genus of fly midgut-dwelling parasites that bear two flagella in contrast to other trypanosomatids, which are unambiguously uniflagellate.</jats:p>
</jats:sec><jats:sec>
<jats:title>Results</jats:title>
<jats:p><jats:italic>Vickermania</jats:italic> has an odd cell cycle, in which shortly after the division the uniflagellate cell starts growing a new flagellum attached to the old one and preserves their contact until the late cytokinesis. The flagella connect to each other throughout their whole length and carry a peculiar seizing structure with a paddle-like apex and two lateral extensions at their tip. In contrast to typical trypanosomatids, which attach to the insect host’s intestinal wall, <jats:italic>Vickermania</jats:italic> is separated from it by a continuous peritrophic membrane and resides freely in the fly midgut lumen.</jats:p>
</jats:sec><jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>We propose that <jats:italic>Vickermania</jats:italic> developed a survival strategy that relies on constant movement preventing discharge from the host gut due to intestinal peristalsis. Since these parasites cannot attach to the midgut wall, they were forced to shorten the period of impaired motility when two separate flagella in dividing cells interfere with each other. The connection between the flagella ensures their coordinate movement until the separation of the daughter cells. We propose that <jats:italic>Trypanosoma brucei</jats:italic>, a severe human pathogen, during its development in the tsetse fly midgut faces the same conditions and follows the same strategy as <jats:italic>Vickermania</jats:italic> by employing an analogous adaptation, the flagellar connector.</jats:p>
</jats:sec>
<jats:title>Background</jats:title>
<jats:p>The family Trypanosomatidae encompasses parasitic flagellates, some of which cause serious vector-transmitted diseases of humans and domestic animals. However, insect-restricted parasites represent the ancestral and most diverse group within the family. They display a range of unusual features and their study can provide insights into the biology of human pathogens. Here we describe <jats:italic>Vickermania</jats:italic>, a new genus of fly midgut-dwelling parasites that bear two flagella in contrast to other trypanosomatids, which are unambiguously uniflagellate.</jats:p>
</jats:sec><jats:sec>
<jats:title>Results</jats:title>
<jats:p><jats:italic>Vickermania</jats:italic> has an odd cell cycle, in which shortly after the division the uniflagellate cell starts growing a new flagellum attached to the old one and preserves their contact until the late cytokinesis. The flagella connect to each other throughout their whole length and carry a peculiar seizing structure with a paddle-like apex and two lateral extensions at their tip. In contrast to typical trypanosomatids, which attach to the insect host’s intestinal wall, <jats:italic>Vickermania</jats:italic> is separated from it by a continuous peritrophic membrane and resides freely in the fly midgut lumen.</jats:p>
</jats:sec><jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>We propose that <jats:italic>Vickermania</jats:italic> developed a survival strategy that relies on constant movement preventing discharge from the host gut due to intestinal peristalsis. Since these parasites cannot attach to the midgut wall, they were forced to shorten the period of impaired motility when two separate flagella in dividing cells interfere with each other. The connection between the flagella ensures their coordinate movement until the separation of the daughter cells. We propose that <jats:italic>Trypanosoma brucei</jats:italic>, a severe human pathogen, during its development in the tsetse fly midgut faces the same conditions and follows the same strategy as <jats:italic>Vickermania</jats:italic> by employing an analogous adaptation, the flagellar connector.</jats:p>
</jats:sec>
George, Emma E.; Husnik, Filip; Tashyreva, Daria; Prokopchuk, Galina; Horák, Aleš; Kwong, Waldan K.; Lukeš, Julius; Keeling, Patrick J.
Highly Reduced Genomes of Protist Endosymbionts Show Evolutionary Convergence Journal Article
In: Current Biology, vol. 30, no. 5, pp. 925–933.e3, 2020, ISSN: 0960-9822.
@article{George2020,
title = {Highly Reduced Genomes of Protist Endosymbionts Show Evolutionary Convergence},
author = {Emma E. George and Filip Husnik and Daria Tashyreva and Galina Prokopchuk and Aleš Horák and Waldan K. Kwong and Julius Lukeš and Patrick J. Keeling},
doi = {10.1016/j.cub.2019.12.070},
issn = {0960-9822},
year = {2020},
date = {2020-03-00},
urldate = {2020-03-00},
journal = {Current Biology},
volume = {30},
number = {5},
pages = {925--933.e3},
publisher = {Elsevier BV},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2019
Prokopchuk, Galina; Tashyreva, Daria; Yabuki, Akinori; Horák, Aleš; Masařová, Petra; Lukeš, Julius
Morphological, Ultrastructural, Motility and Evolutionary Characterization of Two New Hemistasiidae Species Journal Article
In: Protist, vol. 170, no. 3, pp. 259–282, 2019, ISSN: 1434-4610.
@article{Prokopchuk2019,
title = {Morphological, Ultrastructural, Motility and Evolutionary Characterization of Two New Hemistasiidae Species},
author = {Galina Prokopchuk and Daria Tashyreva and Akinori Yabuki and Aleš Horák and Petra Masařová and Julius Lukeš},
doi = {10.1016/j.protis.2019.04.001},
issn = {1434-4610},
year = {2019},
date = {2019-07-00},
urldate = {2019-07-00},
journal = {Protist},
volume = {170},
number = {3},
pages = {259--282},
publisher = {Elsevier BV},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2018
Tashyreva, Daria; Prokopchuk, Galina; Votýpka, Jan; Yabuki, Akinori; Horák, Aleš; Lukeš, Julius
Life Cycle, Ultrastructure, and Phylogeny of New Diplonemids and Their Endosymbiotic Bacteria Journal Article
In: mBio, vol. 9, no. 2, 2018, ISSN: 2150-7511.
@article{Tashyreva2018,
title = {Life Cycle, Ultrastructure, and Phylogeny of New Diplonemids and Their Endosymbiotic Bacteria},
author = {Daria Tashyreva and Galina Prokopchuk and Jan Votýpka and Akinori Yabuki and Aleš Horák and Julius Lukeš},
editor = {Joseph Heitman},
doi = {10.1128/mbio.02447-17},
issn = {2150-7511},
year = {2018},
date = {2018-05-02},
urldate = {2018-05-02},
journal = {mBio},
volume = {9},
number = {2},
publisher = {American Society for Microbiology},
abstract = {<jats:title>ABSTRACT</jats:title>
<jats:p>
Diplonemids represent a hyperdiverse and abundant yet poorly studied group of marine protists. Here we describe two new members of the genus
<jats:italic>Diplonema</jats:italic>
(Diplonemea, Euglenozoa),
<jats:italic>Diplonema japonicum</jats:italic>
sp. nov. and
<jats:italic>Diplonema aggregatum</jats:italic>
sp. nov., based on life cycle, morphology, and 18S rRNA gene sequences. Along with euglenozoan apomorphies, they contain several unique features. Their life cycle is complex, consisting of a trophic stage that is, following the depletion of nutrients, transformed into a sessile stage and subsequently into a swimming stage. The latter two stages are characterized by the presence of tubular extrusomes and the emergence of a paraflagellar rod, the supportive structure of the flagellum, which is prominently lacking in the trophic stage. These two stages also differ dramatically in motility and flagellar size. Both diplonemid species host endosymbiotic bacteria that are closely related to each other and constitute a novel branch within
<jats:italic>Holosporales</jats:italic>
, for which a new genus, “
<jats:italic>Candidatus</jats:italic>
Cytomitobacter” gen. nov., has been established. Remarkably, the number of endosymbionts in the cytoplasm varies significantly, as does their localization within the cell, where they seem to penetrate the mitochondrion, a rare occurrence.
</jats:p>
<jats:p>
<jats:bold>IMPORTANCE</jats:bold>
We describe the morphology, behavior, and life cycle of two new
<jats:italic>Diplonema</jats:italic>
species that established a relationship with two
<jats:italic>Holospora</jats:italic>
-like bacteria in the first report of an endosymbiosis in diplonemids. Both endosymbionts reside in the cytoplasm and the mitochondrion, which establishes an extremely rare case. Within their life cycle, the diplonemids undergo transformation from a trophic to a sessile and eventually a highly motile swimming stage. These stages differ in several features, such as the presence or absence of tubular extrusomes and a paraflagellar rod, along with the length of the flagella. These morphological and behavioral interstage differences possibly reflect distinct functions in dispersion and invasion of the host and/or prey and may provide novel insight into the virtually unknown function of diplonemids in the oceanic ecosystem.
</jats:p>},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
<jats:title>ABSTRACT</jats:title>
<jats:p>
Diplonemids represent a hyperdiverse and abundant yet poorly studied group of marine protists. Here we describe two new members of the genus
<jats:italic>Diplonema</jats:italic>
(Diplonemea, Euglenozoa),
<jats:italic>Diplonema japonicum</jats:italic>
sp. nov. and
<jats:italic>Diplonema aggregatum</jats:italic>
sp. nov., based on life cycle, morphology, and 18S rRNA gene sequences. Along with euglenozoan apomorphies, they contain several unique features. Their life cycle is complex, consisting of a trophic stage that is, following the depletion of nutrients, transformed into a sessile stage and subsequently into a swimming stage. The latter two stages are characterized by the presence of tubular extrusomes and the emergence of a paraflagellar rod, the supportive structure of the flagellum, which is prominently lacking in the trophic stage. These two stages also differ dramatically in motility and flagellar size. Both diplonemid species host endosymbiotic bacteria that are closely related to each other and constitute a novel branch within
<jats:italic>Holosporales</jats:italic>
, for which a new genus, “
<jats:italic>Candidatus</jats:italic>
Cytomitobacter” gen. nov., has been established. Remarkably, the number of endosymbionts in the cytoplasm varies significantly, as does their localization within the cell, where they seem to penetrate the mitochondrion, a rare occurrence.
</jats:p>
<jats:p>
<jats:bold>IMPORTANCE</jats:bold>
We describe the morphology, behavior, and life cycle of two new
<jats:italic>Diplonema</jats:italic>
species that established a relationship with two
<jats:italic>Holospora</jats:italic>
-like bacteria in the first report of an endosymbiosis in diplonemids. Both endosymbionts reside in the cytoplasm and the mitochondrion, which establishes an extremely rare case. Within their life cycle, the diplonemids undergo transformation from a trophic to a sessile and eventually a highly motile swimming stage. These stages differ in several features, such as the presence or absence of tubular extrusomes and a paraflagellar rod, along with the length of the flagella. These morphological and behavioral interstage differences possibly reflect distinct functions in dispersion and invasion of the host and/or prey and may provide novel insight into the virtually unknown function of diplonemids in the oceanic ecosystem.
</jats:p>
<jats:p>
Diplonemids represent a hyperdiverse and abundant yet poorly studied group of marine protists. Here we describe two new members of the genus
<jats:italic>Diplonema</jats:italic>
(Diplonemea, Euglenozoa),
<jats:italic>Diplonema japonicum</jats:italic>
sp. nov. and
<jats:italic>Diplonema aggregatum</jats:italic>
sp. nov., based on life cycle, morphology, and 18S rRNA gene sequences. Along with euglenozoan apomorphies, they contain several unique features. Their life cycle is complex, consisting of a trophic stage that is, following the depletion of nutrients, transformed into a sessile stage and subsequently into a swimming stage. The latter two stages are characterized by the presence of tubular extrusomes and the emergence of a paraflagellar rod, the supportive structure of the flagellum, which is prominently lacking in the trophic stage. These two stages also differ dramatically in motility and flagellar size. Both diplonemid species host endosymbiotic bacteria that are closely related to each other and constitute a novel branch within
<jats:italic>Holosporales</jats:italic>
, for which a new genus, “
<jats:italic>Candidatus</jats:italic>
Cytomitobacter” gen. nov., has been established. Remarkably, the number of endosymbionts in the cytoplasm varies significantly, as does their localization within the cell, where they seem to penetrate the mitochondrion, a rare occurrence.
</jats:p>
<jats:p>
<jats:bold>IMPORTANCE</jats:bold>
We describe the morphology, behavior, and life cycle of two new
<jats:italic>Diplonema</jats:italic>
species that established a relationship with two
<jats:italic>Holospora</jats:italic>
-like bacteria in the first report of an endosymbiosis in diplonemids. Both endosymbionts reside in the cytoplasm and the mitochondrion, which establishes an extremely rare case. Within their life cycle, the diplonemids undergo transformation from a trophic to a sessile and eventually a highly motile swimming stage. These stages differ in several features, such as the presence or absence of tubular extrusomes and a paraflagellar rod, along with the length of the flagella. These morphological and behavioral interstage differences possibly reflect distinct functions in dispersion and invasion of the host and/or prey and may provide novel insight into the virtually unknown function of diplonemids in the oceanic ecosystem.
</jats:p>
Tashyreva, Daria; Prokopchuk, Galina; Yabuki, Akinori; Kaur, Binnypreet; Faktorová, Drahomíra; Votýpka, Jan; Kusaka, Chiho; Fujikura, Katsunori; Shiratori, Takashi; Ishida, Ken-Ichiro; Horák, Aleš; Lukeš, Julius
Phylogeny and Morphology of New Diplonemids from Japan Journal Article
In: Protist, vol. 169, no. 2, pp. 158–179, 2018, ISSN: 1434-4610.
@article{Tashyreva2018b,
title = {Phylogeny and Morphology of New Diplonemids from Japan},
author = {Daria Tashyreva and Galina Prokopchuk and Akinori Yabuki and Binnypreet Kaur and Drahomíra Faktorová and Jan Votýpka and Chiho Kusaka and Katsunori Fujikura and Takashi Shiratori and Ken-Ichiro Ishida and Aleš Horák and Julius Lukeš},
doi = {10.1016/j.protis.2018.02.001},
issn = {1434-4610},
year = {2018},
date = {2018-04-00},
urldate = {2018-04-00},
journal = {Protist},
volume = {169},
number = {2},
pages = {158--179},
publisher = {Elsevier BV},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2017
Kostygov, Alexei Y.; Butenko, Anzhelika; Nenarokova, Anna; Tashyreva, Daria; Flegontov, Pavel; Lukeš, Julius; Yurchenko, Vyacheslav
Genome of Ca. Pandoraea novymonadis, an Endosymbiotic Bacterium of the Trypanosomatid Novymonas esmeraldas Journal Article
In: Front. Microbiol., vol. 8, 2017, ISSN: 1664-302X.
@article{Kostygov2017,
title = {Genome of Ca. Pandoraea novymonadis, an Endosymbiotic Bacterium of the Trypanosomatid Novymonas esmeraldas},
author = {Alexei Y. Kostygov and Anzhelika Butenko and Anna Nenarokova and Daria Tashyreva and Pavel Flegontov and Julius Lukeš and Vyacheslav Yurchenko},
doi = {10.3389/fmicb.2017.01940},
issn = {1664-302X},
year = {2017},
date = {2017-10-04},
urldate = {2017-10-04},
journal = {Front. Microbiol.},
volume = {8},
publisher = {Frontiers Media SA},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Bégin, Paschale Noël; Lebedeva, Lyudmila; Tashyreva, Daria; Velazquez, David; Blaen, Phillip J.
Future priorities for Arctic freshwater science from the perspective of early career researchers Journal Article
In: Arctic Science, 2017, ISSN: 2368-7460.
@article{Bégin2017,
title = {Future priorities for Arctic freshwater science from the perspective of early career researchers},
author = {Paschale Noël Bégin and Lyudmila Lebedeva and Daria Tashyreva and David Velazquez and Phillip J. Blaen},
doi = {10.1139/as-2016-0028},
issn = {2368-7460},
year = {2017},
date = {2017-07-06},
urldate = {2017-07-06},
journal = {Arctic Science},
publisher = {Canadian Science Publishing},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2016
Tashyreva, Daria; Elster, Josef
Annual Cycles of Two Cyanobacterial Mat Communities in Hydro-Terrestrial Habitats of the High Arctic Journal Article
In: Microb Ecol, vol. 71, no. 4, pp. 887–900, 2016, ISSN: 1432-184X.
@article{Tashyreva2016,
title = {Annual Cycles of Two Cyanobacterial Mat Communities in Hydro-Terrestrial Habitats of the High Arctic},
author = {Daria Tashyreva and Josef Elster},
doi = {10.1007/s00248-016-0732-x},
issn = {1432-184X},
year = {2016},
date = {2016-05-00},
urldate = {2016-05-00},
journal = {Microb Ecol},
volume = {71},
number = {4},
pages = {887--900},
publisher = {Springer Science and Business Media LLC},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2015
Tashyreva, Daria; Elster, Josef
Effect of nitrogen starvation on desiccation tolerance of Arctic Microcoleus strains (cyanobacteria) Journal Article
In: Front. Microbiol., vol. 6, 2015, ISSN: 1664-302X.
@article{Tashyreva2015b,
title = {Effect of nitrogen starvation on desiccation tolerance of Arctic Microcoleus strains (cyanobacteria)},
author = {Daria Tashyreva and Josef Elster},
doi = {10.3389/fmicb.2015.00278},
issn = {1664-302X},
year = {2015},
date = {2015-04-08},
urldate = {2015-04-08},
journal = {Front. Microbiol.},
volume = {6},
publisher = {Frontiers Media SA},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2013
Tashyreva, Daria; Elster, Josef; Billi, Daniela
In: PLoS ONE, vol. 8, no. 2, 2013, ISSN: 1932-6203.
@article{Tashyreva2013,
title = {A Novel Staining Protocol for Multiparameter Assessment of Cell Heterogeneity in Phormidium Populations (Cyanobacteria) Employing Fluorescent Dyes},
author = {Daria Tashyreva and Josef Elster and Daniela Billi},
editor = {Lucas J. Stal},
doi = {10.1371/journal.pone.0055283},
issn = {1932-6203},
year = {2013},
date = {2013-02-20},
urldate = {2013-02-20},
journal = {PLoS ONE},
volume = {8},
number = {2},
publisher = {Public Library of Science (PLoS)},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
2012
Tashyreva, Daria; Elster, Josef
Production of Dormant Stages and Stress Resistance of Polar Cyanobacteria Book Chapter
In: Cellular Origin, Life in Extreme Habitats and Astrobiology, pp. 367–386, Springer Netherlands, 2012, ISBN: 9789400749665.
@inbook{Tashyreva2012,
title = {Production of Dormant Stages and Stress Resistance of Polar Cyanobacteria},
author = {Daria Tashyreva and Josef Elster},
doi = {10.1007/978-94-007-4966-5_21},
isbn = {9789400749665},
year = {2012},
date = {2012-00-00},
urldate = {2012-00-00},
booktitle = {Cellular Origin, Life in Extreme Habitats and Astrobiology},
pages = {367--386},
publisher = {Springer Netherlands},
keywords = {},
pubstate = {published},
tppubtype = {inbook}
}