dr Karolina Grabowska-Grucza

@article{Grabowska_Grucza_2023,

title = {Relationships between Legionella and Aeromonas spp. and associated lake bacterial communities across seasonal changes in an anthropogenic eutrophication gradient},

author = {Karolina Grabowska-Grucza and Bartosz Kiersztyn},

url = {https://doi.org/10.1038%2Fs41598-023-43234-3},

doi = {10.1038/s41598-023-43234-3},

year  = {2023},

date = {2023-10-01},

urldate = {2023-10-01},

journal = {Scientific Reports},

volume = {13},

number = {1},

pages = {17076},

publisher = {Springer Science and Business Media LLC},

abstract = {Anthropogenic eutrophication of lakes threatens their homeostasis and carries an increased risk of development of potentially pathogenic microorganisms. In this paper we show how eutrophication affects seasonal changes in the taxonomic structure of bacterioplankton and whether these changes are associated with the relative abundance of pathogenic bacteria of the genera Legionella and Aeromonas. The subject of the study was a unique system of interconnected lakes in northern Poland (Great Masurian Lakes system), characterized by the presence of eutrophic gradient. We found that the taxonomic structure of the bacterial community in eutrophic lakes was significantly season dependent. No such significant seasonal changes were observed in meso-eutrophic lakes. We found that there is a specific taxonomic composition of bacteria associated with the occurrence of Legionella spp. The highest positive significant correlations were found for families Pirellulaceae, Mycobacteriaceae and Gemmataceae. The highest negative correlations were found for the families Sporichthyaceae, Flavobacteriaceae, the uncultured families of class Verrucomicrobia and Chitinophagaceae. We used also an Automatic Neural Network model to estimate the relative abundance of Legionella spp. based on the relative abundance of dominant bacterial families. In the case of Aeromonas spp. we did not find a clear relationship with bacterial communities inhabiting lakes of different trophic state. Our research has shown that anthropogenic eutrophication causes significant changes in the taxonomic composition of lake bacteria and contributes to an increase in the proportion of potentially pathogenic Legionella spp.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Comparison of \textit{Yersinia enterocolitica} DNA Isolation from Swabs Without Pre-Enrichment on Selective Media With Isolation Preceded by Warm and Cold Enrichment},

author = {Karolina Grabowska-Grucza and Agata Bancerz-Kisiel},

doi = {10.1089/fpd.2021.0074},

issn = {1535-3141},

year  = {2022},

date = {2022-01-01},

journal = {Foodborne Pathogens and Disease},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Grabowska_Grucza_2022,

title = {Impact of increasing temperature on the taxonomic and metabolic structure of bacterial communities in a global warming context},

author = {K Grabowska-Grucza and A Bukowska and W Siuda and RJ Chr ó and B Kiersztyn},

url = {https://doi.org/10.3354%2Fame01988},

doi = {10.3354/ame01988},

year  = {2022},

date = {2022-01-01},

journal = {Aquatic Microbial Ecology},

publisher = {Inter-Research Science Center},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Siuda2020,

title = {Trophic state, eutrophication, and the threats for water quality of the great mazurian lake system},

author = {Waldemar Siuda and Karolina Grabowska and Tomasz Kaliński and Bartosz Kiersztyn and Ryszard J Chróst},

doi = {10.1007/978-3-030-12123-5_12},

issn = {1867979X},

year  = {2020},

date = {2020-01-01},

journal = {Handbook of Environmental Chemistry},

volume = {86},

pages = {231-260},

publisher = {Springer Verlag},

abstract = {One of the greatest threats to water quality is accelerated eutrophication, resulting from human activity, like the high intensity of tourism, surface runoffs from fertilized fields, and municipal pollution. Water eutrophication manifests as excessive growth of phytoplankton caused by overabundant nitrogen, phosphorus, and other nutrient supply which causes deterioration of water quality related to the amount of bacterial biomass in eutrophicated water reservoirs. The Great Mazurian Lake System (GMLS) is a chain of lakes located in mesoregion of the Great Mazurian Lakes in the Northeastern Poland. All lakes of the GMLS are connected by natural or artificial channels built in the eighteenth and nineteenth centuries and nowadays create widely spilled, long (the easiest route from northern to southern edge is about 110 km) gutter unique on the scale of the continent. The lakes of GMLS are of glacial origin. During the last five decades, all lakes of the GMLS passed different levels of eutrophication, thus significantly changing their trophic states. This report describes past and present trophic conditions of lakes of GMLS and analyzes environmental factors responsible for eutrophication of their waters. Eutrophication processes are not only responsible for high nutrients levels in lakes, extensive growth of phytoplankton biomass and productivity, cyanobacterial predominance, etc., but eutrophication is also responsible and connected to several threats for water quality. Presence of pathogenic bacteria, as well as the potential presence of many antibiotic-resistant bacteria in lakes of the GMLS, is discussed.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Grabowska2019,

title = {Presence and identification of Legionella and Aeromonas spp. in the Great Masurian Lakes system in the context of eutrophication},

author = {Karolina Grabowska and Aleksandra Bukowska and Tomasz Kaliński and Bartosz Kiersztyn and Waldemar Siuda and Ryszard J Chróst},

doi = {10.4081/jlimnol.2019.1924},

issn = {1129-5767},

year  = {2019},

date = {2019-01-01},

journal = {Journal of Limnology},

abstract = {Growing anthropopressure over the last several decades has resulted in rapid progressive eutrophication of the Great Masurian Lakes (GML) system located in northeastern Poland. In our studies, we investigated whether there is a relationship between the occurrence of pathogenic bacteria: Legionella spp. and Aeromonas spp., not explored so far in the waters of GML system, and the trophic status of the studied lakes. The GML system of glacial origin includes lakes connected by natural and artificial channels, and it extends from north to south for approximately 100 kilometers. Water samples were taken during the summer, subsequently spring and autumn seasons from 15 lakes in land-water ecotones. At all sampling sites, basic in situ measurements of physicochemical parameters were recorded. The amounts of chlorophyll a, nitrogen, phosphorus, dissolved organic carbon were also measured. The trophic state index (TSI) of the sampling sites was also estimated. The real-time PCR technique enabled the determination of the presence and abundance of Legionella spp. and Aeromonas spp. The results clearly showed that several environmental water quality parameters, associated with eutrophication, and among them: nitrogen, phosphorus, chlorophyll, ammonium concentration, conductivity, turbidity, water transparency, highly affected the presence and abundance of the detected pathogenic bacteria in the studied lakes. Special attention should be paid to the high impact of water eutrophication on the number of pathogenic microorganisms, which result both from human activities in lakes and climate change.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Kiersztyn2019,

title = {Structural and functional microbial diversity along a eutrophication gradient of interconnected lakes undergoing anthropopressure},

author = {Bartosz Kiersztyn and Ryszard Chróst and Tomasz Kaliński and Waldemar Siuda and Aleksandra Bukowska and Grzegorz Kowalczyk and Karolina Grabowska},

doi = {10.1038/s41598-019-47577-8},

issn = {2045-2322},

year  = {2019},

date = {2019-01-01},

journal = {Scientific Reports},

volume = {9},

publisher = {Springer Science and Business Media LLC},

abstract = {We present the results of an analysis of the 16S rRNA-based taxonomical structure of bacteria together with an analysis of carbon source utilization ability using EcoPlate (Biolog, USA) metabolic fingerprinting assessment against the backdrop of physicochemical parameters in fifteen interconnected lakes. The lakes exhibit a wide spectrum of trophic gradients and undergo different intensities of anthropopressure. Sequences of V3-V4 16S rRNA genes binned by taxonomic assignment to family indicated that bacterial communities in the highly eutrophicated lakes were distinctly different from the bacterial communities in the meso-eutrophic lakes (ANOSIM r = 0.99},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{,

title = {Characterization of \textit{Yersinia enterocolitica} strains potentially virulent for humans and animals in river water},

author = {E Terech-Majewska and J Pajdak and A Platt-Samoraj and A Szczerba-Turek and A Bancerz-Kisiel and K Grabowska},

url = {http://doi.wiley.com/10.1111/jam.13182},

doi = {10.1111/jam.13182},

issn = {13645072},

year  = {2016},

date = {2016-01-01},

journal = {Journal of Applied Microbiology},

volume = {121},

pages = {554-560},

keywords = {},

pubstate = {published},

tppubtype = {article}

}