dr Ivan García-Cunchillos

@article{10.3897/imafungus.16.159960,

title = {First insights into the phylogeny of the order Cribrariales (Amoebozoa, Myxomycetes), with the definite exclusion of the genus Enteridium},

author = {Juan Carlos Zamora and Daniel Rodrigues and Iván García-Cunchillos and Carlos Lado},

url = {https://doi.org/10.3897/imafungus.16.159960},

doi = {10.3897/imafungus.16.159960},

issn = {2210-6340},

year  = {2025},

date = {2025-10-02},

urldate = {2025-01-01},

journal = {IMA Fungus},

volume = {16},

pages = {e159960},

publisher = {Pensoft Publishers},

abstract = {The order Cribrariales is among the least studied higher groups in the Myxomycetes, with numerous taxonomic problems and scarce molecular data available in public databases. Of the three genera currently accepted, viz. Cribraria, Lindbladia, and Enteridium, the last one shows a set of morphological characters clearly disagreeing with the two former ones. Using a representative sampling and two unlinked loci (nuclear and mitochondrial SSU), we assessed the phylogenetic relationships in the bright-spored Myxomycetes (Lucisporomycetidae) and concluded that the genus Enteridium must be excluded from the order Cribrariales and placed instead within the order Trichiales, family Dianemataceae. We provide detailed explanations of why this genus has been misclassified in previous studies, and define its morphological and molecular boundaries, performing two necessary new combinations. On the other hand, the phylogeny of the order Cribrariales s.str. shows three main lineages that are distinguished as three subgenera, viz. C. subg. Cribraria, C. subg. Dictydium, and C. subg. Ionokylix subg. nov., the first one including the genus Lindbladia deeply nested and therefore treated as a heterotypic synonym of Cribraria.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{10.3897/imafungus.16.159960b,

title = {Complete mitochondrial genomes of the Prototheca genus: comparative genomics and evolutionary insights},

author = {Daniel Wibberg and Zofia Bakuła and Iván García-Cunchillos and Jan Gawor and Robert Gromadka and Anna Karnkowska and Jacek Bielecki and Tomasz Jagielski},

url = {https://doi.org/10.1186/s12864-025-11952-9},

doi = {10.1186/s12864-025-11952-9},

issn = {1471-2164},

year  = {2025},

date = {2025-08-21},

urldate = {2025-01-01},

journal = {BMC Genomics},

volume = {26},

number = {1},

pages = {766},

publisher = {Springer Nature},

abstract = {Prototheca (Chlorophyta: Trebouxiophyceae) is a genus of non-photosynthetic microalgae that causes increasingly frequent infections in both humans and animals, collectively referred to as protothecosis The genetic landscape of the Prototheca algae has remained largely uncharted until recent advances in sequencing and genomics. In this study, a combination of Illumina and Oxford Nanopore technologies was employed for sequencing of 18 mitochondrial genomes, representing all currently recognized Prototheca species.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{nokey,

title = {Protecting stable biological nomenclatural systems enables universal communication: A collective international appeal},

author = {Pedro Jiménez-Mejías and Saúl Manzano and Vinita Gowda and Frank-Thorsten Krell and Mei-Ying Lin and Santiago Martín-Bravo and Laura Martín-Torrijos and Nieto Feliner, Gonzalo and Sergei L Mosyakin and Robert F C Naczi and Carmen Acedo and Inés Álvarez and Jorge V Crisci and Luceño Garcés, Modesto and John Manning and Moreno Saiz, Juan Carlos and A Muthama Muasya and Ricarda Riina and Sánchez Meseguer, Andrea and Daniel Sánchez-Mata and additional 1543 coauthors},

url = {https://academic.oup.com/bioscience/advance-article/doi/10.1093/biosci/biae043/7696204},

doi = {10.1093/biosci/biae043},

year  = {2024},

date = {2024-06-19},

urldate = {2024-06-19},

journal = {BioScience},

pages = {biae043},

abstract = {The fundamental value of universal nomenclatural systems in biology is that they enable unambiguous scientific communication. However, the stability of these systems is threatened by recent discussions asking for a fairer nomenclature, raising the possibility of bulk revision processes for “inappropriate”names. It is evident that such proposals come from very deep feelings, but we show how they can irreparably damage the foundation of biological communication and, in turn, the sciences that depend on it. There are four essential consequences of objective codes of nomenclature: universality, stability, neutrality, and transculturality. These codes provide fair and impartial guides to the principles governing biological nomenclature and allow unambiguous universal communication in biology. Accordingly, no subjective proposals should be allowed to undermine them.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{https://doi.org/10.1111/1462-2920.16606,

title = {How good are global DNA-based environmental surveys for detecting all protist diversity? Arcellinida as an example of biased representation},

author = {Fernando Useros and Iván García-Cunchillos and Nicolas Henry and Cédric Berney and Enrique Lara},

url = {https://enviromicro-journals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.16606},

doi = {https://doi.org/10.1111/1462-2920.16606},

year  = {2024},

date = {2024-03-20},

urldate = {2024-01-01},

journal = {Environmental Microbiology},

volume = {26},

number = {3},

pages = {e16606},

abstract = {Abstract Metabarcoding approaches targeting microeukaryotes have deeply changed our vision of protist environmental diversity. The public repository EukBank consists of 18S v4 metabarcodes from 12,672 samples worldwide. To estimate how far this database provides a reasonable overview of all eukaryotic diversity, we used Arcellinida (lobose testate amoebae) as a case study. We hypothesised that (1) this approach would allow the discovery of unexpected diversity, but also that (2) some groups would be underrepresented because of primer/sequencing biases. Most of the Arcellinida sequences appeared in freshwater and soil, but their abundance and diversity appeared underrepresented. Moreover, 84% of ASVs belonged to the suborder Phryganellina, a supposedly species-poor clade, whereas the best-documented suborder (Glutinoconcha, 600 described species) was only marginally represented. We explored some possible causes of these biases. Mismatches in the primer-binding site seem to play a minor role. Excessive length of the target region could explain some of these biases, but not all. There must be some other unknown factors involved. Altogether, while metabarcoding based on ribosomal genes remains a good first approach to document microbial eukaryotic clades, alternative approaches based on other genes or sequencing techniques must be considered for an unbiased picture of the diversity of some groups.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{https://doi.org/10.11646/phytotaxa.624.1.1,

title = {Diversity of Myxomycetes from Peru Part III: The high Andes and the altiplano},

author = {Italo Treviño-Zevallos and Iván García-Cunchillos and Diana Wrigley de Basanta and Carlos Lado},

url = {https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.624.1.1},

doi = {https://doi.org/10.11646/phytotaxa.624.1.1},

year  = {2023},

date = {2023-11-03},

urldate = {2023-01-01},

journal = {Phytotaxa},

volume = {624},

number = {1},

pages = {1-92},

abstract = {The results obtained from a survey for Myxomycetes in the Peruvian Andes (between 3,000 and 5,000 m a.s.l.) are reported. This survey is based on 3,352 identifiable collections of myxomycetes, resulting from sampling carried out during six field expeditions of the Myxotropic Project, from moist chamber culture and the revision of deposited specimens in the HSP herbarium. In total, 178 taxa representing 31 genera were recorded. Ten species are considered new records for South America or the Neotropics, and 53 additional new records for Peru are reported. Notes and SEM micrographs of relevant species are included. The best-represented order was Physarales with 55% (99 species), followed by Trichiales (43 species), Stemonitidales (21 species), Cribrariales (14 species), and the least represented was Echinosteliales (1 species). The most diverse genera were Physarum and Didymium, with 43 and 24 spp., respectively. The most abundant species were Didymium nigrisporum, with 336 collections and Didymium difforme, with 156 collections. These data, together with those obtained at other elevations in the country and in the Southern Andes, confirm the high diversity of myxomycetes of Peru, show the biological value of the Andes Cordillera and highlight the vital role that it plays in the distribution of these microorganisms. The study confirms the striking differences between the species assemblages in different vegetation belts of the tropical Andes, with only 18% of the species recovered in all three studied areas in Peru.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{GARCIACUNCHILLOS2022107609,

title = {Phylogeny and evolution of morphological structures in a highly diverse lineage of fruiting-body-forming amoebae, order Trichiales (Myxomycetes, Amoebozoa)},

author = {Iván García-Cunchillos and Juan Carlos Zamora and Martin Ryberg and Carlos Lado},

url = {https://www.sciencedirect.com/science/article/pii/S1055790322002226},

doi = {https://doi.org/10.1016/j.ympev.2022.107609},

issn = {1055-7903},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {Molecular Phylogenetics and Evolution},

volume = {177},

pages = {107609},

abstract = {Early phylogenetic studies refuted most previous assumptions concerning the evolution of the morphological traits in the fruiting bodies of the order Trichiales and did not detect discernible evolutionary patterns, yet they were based on a limited number of species. We infer a new Trichiales phylogeny based on three independently inherited genetic regions (nuclear and mitochondrial), with a fair taxonomic sampling encompassing its broad diversity. Besides, we study the evolutionary history of some key morphological characters. According to the new phylogeny, most fruiting body traits in Trichiales systematics do not represent exclusive synapomorphies or autapomorphies for most monophyletic groups. Instead, the evolution of the features derived from the peridium, stalk, capillitium, and spores showed intricate patterns, and character state transitions occurred rather within- than between clades. Thus, we should consider other evolutionary scenarios instead of assuming the homology of some characters. According to these results, we propose a new classification of Trichiales, including the creation of a new genus, Gulielmina, the resurrection of the family Dictydiaethaliaceae and the genus Ophiotheca, and the proposal of 13 new combinations for species of the genera Arcyria (1), Hemitrichia (2), Ophiotheca (2), Oligonema (4), Gulielmina (3), and Perichaena (1).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articlee,

title = {New records of Myxomycetes (Amoebozoa) from the tropical Andes},

author = {Italo Francisco Zevallos and Iván García-Cunchillos and Carlos Lado},

doi = {10.11646/phytotaxa.522.3.6},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Phytotaxa},

volume = {522},

pages = {231-239},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{GARCIACUNCHILLOS2021125839,

title = {Spore ultrastructural features and significance of their diverse ornamental elements in the evolutionary history of the order Trichiales (Myxomycetes, Amoebozoa)},

author = {Iván García-Cunchillos and Belén Estébanez and Carlos Lado},

url = {https://www.sciencedirect.com/science/article/pii/S0932473921000742},

doi = {https://doi.org/10.1016/j.ejop.2021.125839},

issn = {0932-4739},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {European Journal of Protistology},

volume = {81},

pages = {125839},

abstract = {Spores are the dispersal and reproductive units in Myxomycetes, and their ornamentation, usually at the light microscopy resolution limit, is taxonomically meaningful. Here, we analyze with scanning and transmission electron microscopy the spore ultrastructural features in Trichiales, one of the most morphologically diverse orders. In Trichiales, the spore wall consists of two layers, an outer one, including the ornamentation, and an inner layer subdivided into two sections. The diversity of ornamental elements includes verrucae, bacula, pila, muri, and cristae, the two latter creating reticulate patterns. Each of these elements defines a broadly recognized ornamentation type except cristae, from which we differentiate for the first time the cristate reticulate and patched subtypes. Besides, our results point out a previous incorrect classification of the spores of the species Trichia decipiens and T. scabra. Advances in Trichiales phylogeny showed that the capillitium ornamental elements, such as the spirals, do not reflect phylogenetic relationships among the species. The same seems to apply to some of spore ornamentation types, e.g., baculate. Consequently, we may consider the need to combine multiple characters, like the spore and capillitium ornamentation, so that Trichiales systematics better reflect the still cryptic phylogenetic affinities.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{GARCIACUNCHILLOS2021125805,

title = {New Approach to the Ultrastructure of the Capillitium in the Order Trichiales (Myxomycetes, Amoebozoa) and its Phylogenetic Implications},

author = {Iván García-Cunchillos and Belén Estébanez and Carlos Lado},

url = {https://www.sciencedirect.com/science/article/pii/S1434461021000146},

doi = {https://doi.org/10.1016/j.protis.2021.125805},

issn = {1434-4610},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Protist},

volume = {172},

number = {2},

pages = {125805},

abstract = {Myxomycetes constitute one of the major lineages within the supergroup Amoebozoa. At the end of their life cycles, most myxomycetes produce spore-bearing fruiting bodies, in which additional structures develop, like the capillitium, a system of sterile filaments intermingled with the spores. The capillitium is a relevant structure in the taxonomy of the order Trichiales, the target group in this study. However, the introduction of molecular phylogenies in Myxomycetes systematics is challenging our comprehension of this structure. We studied the capillitium of 25 species representing nine Trichiales genera, with both scanning and transmission electron microscopy. In this order, the capillitium showed higher diversity than so far recognized. Thus, we distinguished and described five capillitium types and two subtypes based on the presence or absence of a lumen and the wall ultrastructure. These types followed the evolutionary history reported in recent phylogenies, although not all of them defined monophyletic groups. Besides, the spiral ornamentation, which most taxonomists considered to have appeared once, occurred in three different capillitium types. The ultrastructural approaches in Myxomycetes systematics enable the reconsideration of their morphological features in the new phylogenetic scenario.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articled,

title = {Nivicolous Trichiales from the austral Andes: unexpected diversity including two new species},

author = {Anna Ronikier and Iván García-Cunchillos and Paulina Janik and Carlos Lado},

doi = {10.1080/00275514.2020.1759978},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-01},

journal = {Mycologia},

volume = {112},

number = {4},

pages = {753-780},

publisher = {Taylor & Francis},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articleb,

title = {Losses of leaf area owing to abiotic stress along the leaf economics spectrum: implications for carbon gain at the branch level},

author = {Sonia Mediavilla and Iván García-Cunchillos and Carmen Andrés-Rivera and Alfonso Escudero},

doi = {10.1007/s00468-018-1656-5},

year  = {2018},

date = {2018-01-01},

urldate = {2018-01-01},

journal = {Trees},

volume = {32},

pages = {1-11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{articlec,

title = {Didymium azorellae, a new myxomycete from cushion plants of cold arid areas of South America},

author = {Diana Wrigley Basanta and Arturo Estrada-Torres and Iván García-Cunchillos and Asunción Cano Echevarría and Carlos Lado},

url = {https://doi.org/10.1080/00275514.2018.1426925},

doi = {10.1080/00275514.2018.1426925},

year  = {2017},

date = {2017-01-01},

urldate = {2017-01-01},

journal = {Mycologia},

volume = {109},

number = {6},

pages = {993-1002},

publisher = {Taylor & Francis},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{article,

title = {Population genetics of the westernmost distribution of the glaciations-surviving black truffle Tuber melanosporum},

author = {Iván García-Cunchillos and Sergio Sánchez and Juan Barriuso and Ernesto Perez-Collazos},

doi = {10.1007/s00572-013-0540-9},

year  = {2013},

date = {2013-01-01},

urldate = {2013-01-01},

journal = {Mycorrhiza},

volume = {24},

keywords = {},

pubstate = {published},

tppubtype = {article}

}