mgr Alicja Okrasińska

@article{https://doi.org/10.1002/ece3.70333,

title = {Ant's Nest as a microenvironment: Distinct Mucoromycota (Fungi) community of the red wood ants' (Formica polyctena) mounds},

author = {Igor Siedlecki and Michał Kochanowski and Julia Pawłowska and Gabriela Reszotnik and Alicja Okrasińska and Marta Wrzosek},

url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/ece3.70333},

doi = {https://doi.org/10.1002/ece3.70333},

year  = {2024},

date = {2024-10-09},

urldate = {2024-10-09},

journal = {Ecology and Evolution},

volume = {14},

number = {10},

pages = {e70333},

abstract = {Abstract Many social insect species build nests, which differ from the surrounding environment and are often occupied by specific organismal communities. These organisms may interact mutualistically or parasitically with the nest-builders, or simply co-occur, being able to survive in these microenvironments. In temperate forests, red wood ants (e.g. Formica polyctena) are known to create distinct, highly developed nests, which consist of large, above-ground mounds, built primarily out of plant matter collected from the forest litter. The microorganismal communities of such mounds remain understudied. As representatives of Mucoromycota fungi commonly engage in the decomposition process of the forest litter, they would be expected to occur in the mounds. However, it is still not known whether the Mucoromycota community of these ants' nests differ from the one of the surrounding forest litter. In order to distinguish mound-associated taxa, we characterized Mucoromycota communities of Formica polyctena mounds and the surrounding forest litter. We sampled four sites, twice in a season. Sampled material was plated on agar media and emerging Mucoromycota colonies were identified based on their morphology. Fungal identification was further confirmed using DNA barcoding. In order to compare described communities, PERMANOVA test and non-metric multidimensional scaling ordinations were used. To distinguish taxa associated with the mounds, multilevel pattern analysis was performed. Our results show that the Mucoromycota community of Formica polyctena's mound differs from the community of the surrounding forest litter. While representatives of Entomortierella lignicola and Absidia cylindrospora clade were found to be associated with the mound environment, representatives of Umbelopsis curvata and Podila verticillata-humilis clade were associated with forest litter, and were rarely present in the mounds. Our findings strongly suggest that the red wood ants' nest is a specific microenvironment in the temperate forest floor, which is a preferred microhabitat for the mound-associated Mucoromycota, possibly adapted to live in proximity to ants.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Abramczyk24,

title = { Mucor thermorhizoides—A New Species from Post-mining Site in Sudety Mountains (Poland).},

author = {Beniamin Abramczyk and Dorota Wiktorowicz and Alicja Okrasińska and Julia Pawłowska},

url = {https://link.springer.com/article/10.1007/s00284-024-03708-7},

doi = {https://doi.org/10.1007/s00284-024-03708-7},

year  = {2024},

date = {2024-06-01},

urldate = {2024-06-01},

journal = {Current Microbiology},

volume = {81},

issue = {201},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Sokolowska23,

title = {What can be lost? Genomic perspective on the lipid metabolism of Mucoromycota},

author = {Blanka Sokołowska and Małgorzata Orłowska and Alicja Okrasińska and Sebastian Piłsyk and Julia Pawłowska and Anna Muszewska},

doi = {https://doi.org/10.1186/s43008-023-00127-4},

year  = {2023},

date = {2023-11-06},

journal = {IMA Fungus},

volume = {14},

issue = {22},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{SIEDLECKI2023,

title = {Discovery of Formicomyces microglobosus gen. et sp. nov. strengthens the hypothesis of independent evolution of ant-associated fungi in Trichomeriaceae},

author = {Igor Siedlecki and Marcin Piątek and Maria Majchrowska and Alicja Okrasińska and Magdalena Owczarek-Kościelniak and Julia Pawłowska},

url = {https://www.sciencedirect.com/science/article/pii/S1878614623001083},

doi = {https://doi.org/10.1016/j.funbio.2023.10.005},

issn = {1878-6146},

year  = {2023},

date = {2023-10-24},

urldate = {2023-10-24},

journal = {Fungal Biology},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{CrousEtAl2023,

title = {Fungal Planet description sheets: 1478–1549},

author = {Crous P.W. and Osieck, E.R. and Shivas, R.G. and Tan, Y.P. and Bishop-Hurley, S.L. and Esteve-Raventós, F. and Larsson, E. and Luangsa-ard, J.J. and Pancorbo, F. and Balashov, S. and Baseia, I.G. and Boekhout, T. and Chandranayaka, S. and Cowan, D.A. and Cruz, R.H.S.F. and Czachura, P. and De la Peña-Lastra, S. and Dovana, F. and Drury, B. and Fell, J. and Flakus, A. and Fotedar, R. and Jurjević, Ž. and Kolecka, A. and Mack, J. and Maggs-Kölling, G. and Mahadevakumar, S. and Mateos, A. and Mongkolsamrit, S. and Noisripoom, W. and Plaza, M. and Overy, D.P. and Piątek, M. and Sandoval-Denis, M. and Vauras, J. and Wingfield, M.J. and Abell, S.E. and Ahmadpour, A. and Akulov, A. and Alavi, F. and Alavi, Z. and Altes, A. and Alvarado, P. and Anand, G. and Ashtekar, N. and Assyov, B. and Banc-Prandi, G. and Barbosa, K.D. and Barreto, G.G. and Bellanger, J.-M. and Bezerra, J.L. and Bhat, D.J. and Bilanski, P. and Bose, T. and Bozok, F. and Chaves, J. and Costa-Rezende, D.H. and Danteswari, C. and Darmostuk, V. and Delgado, G. and Denman, S. and Eichmeier, A. and Etayo, J. and Eyssartier, G. and Faulwetter, S. and Ganga, K.G.G. and Ghosta, Y. and Goh, J. and Góis, J.S. and Gramaje, D. and Granit, L. and Groenewald, M. and Gulden, G. and Gusmão, L.F.P. and Hammerbacher, A. and Heidarian, Z. and Hywel-Jones, N. and Jankowiak, R. and Kaliyaperumal, M. and Kaygusuz, O. and Kezo, K. and Khonsanit, A. and Kumar, S. and Kuo, C.H. and Læssøe, T. and Latha, K.P.D. and Loizides, M. and Luo, S.M. and Maciá-Vicente, J.G. and Manimohan, P. and Marbach, P.A.S. and Marinho, P. and Marney, T.S. and Marques, G. and Martín, M.P. and Miller, A.N. and Mondello, F. and Moreno, G. and Mufeeda, K.T. and Mun, H.Y. and Nau, T. and Nkomo, T. and Okrasińska, A. and Oliveira, J.P.A.F. and Oliveira, R.L. and Ortiz, D.A. and Pawłowska, J. and Pérez-De-Gregorio, M.À. and Podile, A.R. and Portugal, A. and Privitera, N. and Rajeshkumar, K.C. and Rauf, I. and Rian, B. and Rigueiro-Rodríguez, A. and Rivas-Torres, G.F. and Rodriguez-Flakus, P. and Romero-Gordillo, M. and Saar, I. and Saba, M. and Santos, C.D. and Sarma, P.V.S.R.N. and Siquier, J.L. and Sleiman, S. and Spetik, M. and Sridhar, K.R. and Stryjak-Bogacka, M. and Szczepańska, K. and Taşkın, H. and Tennakoon, D.S. and Thanakitpipattana, D. and Trovao, J. and Türkekul, İ. and van Iperen, A.L. and van 't Hof, P. and Vasquez, G. and Visagie, C.M. and Wingfield, B.D. and Wong, P.T.W. and Yang, W.X. and Yarar, M. and Yarden, O. and Yilmaz, N. and Zhang, N. and Zhu, Y.N. and Groenewald, J.Z.},

url = {https://www.ingentaconnect.com/content/nhn/pimj/pre-prints/content-nbc-persoonia-0661#},

doi = {https://doi.org/10.3767/persoonia.2023.50.05},

year  = {2023},

date = {2023-07-03},

urldate = {2023-07-03},

journal = {Persoonia - Molecular Phylogeny and Evolution of Fungi},

volume = {50},

pages = {158-310},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{https://doi.org/10.1111/1462-2920.16007,

title = {Marginal lands and fungi – linking the type of soil contamination with fungal community composition},

author = {Alicja Okrasińska and Przemyslaw Decewicz and Maria Majchrowska and Lukasz Dziewit and Anna Muszewska and Somayeh Dolatabadi and Łukasz Kruszewski and Zuzanna Błocka and Julia Pawłowska},

url = {https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.16007},

doi = {https://doi.org/10.1111/1462-2920.16007},

year  = {2022},

date = {2022-05-31},

urldate = {2022-05-31},

journal = {Environmental Microbiology},

abstract = {Summary Fungi can be found in almost all ecosystems. Some of them can even survive in harsh, anthropogenically transformed environments, such as post-industrial soils. In order to verify how the soil fungal diversity may be changed by pollution, two soil samples from each of the 28 post-industrial sites were collected. Each soil sample was characterized in terms of concentration of heavy metals and petroleum derivatives. To identify soil fungal communities, fungal internal transcribed spacer 2 (ITS2) amplicon was sequenced for each sample using Illumina MiSeq platform. There were significant differences in the community structure and taxonomic diversity among the analysed samples. The highest taxon richness and evenness were observed in the non-polluted sites, and lower numbers of taxa were identified in multi-polluted soils. The presence of monocyclic aromatic hydrocarbons, gasoline and mineral oil was determined as the factors driving the differences in the mycobiome. Furthermore, in the culture-based selection experiment, two main groups of fungi growing on polluted media were identified – generalists able to live in the presence of pollution, and specialists adapted to the usage of BTEX as a sole source of energy. Our selection experiment proved that it is long-term soil contamination that shapes the community, rather than temporary addition of pollutant.

},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{muszewska2021,

title = {Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina},

author = {Anna Muszewska and Alicja Okrasińska and Kamil Steczkiewicz and Olga Drgas and Małgorzata Orłowska and Urszula Perlińska-Lenart and Tamara Aleksandrzak-Piekarczyk and Katarzyna Szatraj and Urszula Zielenkiewicz and Sebastian Piłsyk and Ewa Malc and Piotr Mieczkowski and Joanna S. Kruszewska and Przemysław Bernat and Julia Pawłowska},

url = {https://www.frontiersin.org/article/10.3389/fmicb.2021.636986},

doi = {10.3389/fmicb.2021.636986},

year  = {2021},

date = {2021-02-15},

journal = {Frontiers in Microbiology},

volume = {12},

pages = {239},

abstract = {Mucoromycotina are often considered mainly in pathogenic context but their biology remains understudied. We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e., Umbelopsidales and Mucorales). We selected two Umbelopsis isolates from soil (i.e., U. isabellina, U. vinacea), two soil-derived Mucor isolates (i.e., M. circinatus, M. plumbeus), and two Mucorales representatives with extended proteolytic activity (i.e., Thamnidium elegans and Mucor saturninus). We complement computational genome annotation with experimental characteristics of their digestive capabilities, cell wall carbohydrate composition, and extensive total lipid profiles. These traits inferred from genome composition, e.g., in terms of identified encoded enzymes, are in accordance with experimental results. Finally, we link the presence of associated bacteria with observed characteristics. Thamnidium elegans genome harbors an additional, complete genome of an associated bacterium classified to Paenibacillus sp. This fungus displays multiple altered traits compared to the remaining isolates, regardless of their evolutionary distance. For instance, it has expanded carbon assimilation capabilities, e.g., efficiently degrades carboxylic acids, and has a higher diacylglycerol:triacylglycerol ratio and skewed phospholipid composition which suggests a more rigid cellular membrane. The bacterium can complement the host enzymatic capabilities, alter the fungal metabolism, cell membrane composition but does not change the composition of the cell wall of the fungus. Comparison of early-diverging Umbelopsidales with evolutionary younger Mucorales points at several subtle differences particularly in their carbon source preferences and encoded carbohydrate repertoire. Nevertheless, all tested Mucoromycotina share features including the ability to produce 18:3 gamma-linoleic acid, use TAG as the storage lipid and have fucose as a cell wall component.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{insects12030204,

title = {Chance or Necessity—The Fungi Co−Occurring with Formica polyctena Ants},

author = {Igor Siedlecki and Michał Gorczak and Alicja Okrasińska and Marta Wrzosek},

url = {https://www.mdpi.com/2075-4450/12/3/204},

doi = {10.3390/insects12030204},

year  = {2021},

date = {2021-01-01},

urldate = {2021-01-01},

journal = {Insects},

volume = {12},

number = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Okrasińskae02707-20,

title = {New Endohyphal Relationships between Mucoromycota and Burkholderiaceae Representatives},

author = {Alicja Okrasińska and Aleksandra Bokus and Katarzyna Duk and Aleksandra Gęsiorska and Blanka Sokołowska and Aleksandra Miłobędzka and Marta Wrzosek and Julia Pawłowska},

editor = {Maia Kivisaar},

url = {https://aem.asm.org/content/87/7/e02707-20},

doi = {10.1128/AEM.02707-20},

issn = {0099-2240},

year  = {2021},

date = {2021-01-01},

journal = {Applied and Environmental Microbiology},

volume = {87},

number = {7},

publisher = {American Society for Microbiology Journals},

abstract = {Mucoromycota representatives are known to harbor two types of endohyphal bacteria (EHB)—Burkholderia-related endobacteria (BRE) and Mycoplasma-related endobacteria (MRE). While both BRE and MRE occur in fungi representing all subphyla of Mucoromycota, their distribution is not well studied. Therefore, it is difficult to resolve the evolutionary history of these associations in favor of one of the following two alternative hypotheses explaining their origin: textquotedblleftearly invasiontextquotedblright and textquotedblleftlate invasion.textquotedblright Our main goal was to fill this knowledge gap by surveying Mucoromycota fungi for the presence of EHB. We screened 196 fungal strains from 16 genera using a PCR-based approach to detect bacterial 16S rRNA genes, complemented with fluorescence in situ hybridization (FISH) imaging to confirm the presence of bacteria within the hyphae. We detected Burkholderiaceae in ca. 20% of fungal strains. Some of these bacteria clustered phylogenetically with previously described BRE clades, whereas others grouped with free-living Paraburkholderia. Importantly, the latter were detected in Umbelopsidales, which previously were not known to harbor endobacteria. Our results suggest that this group of EHB is recruited from the environment, supporting the late invasion scenario. This pattern complements the early invasion scenario apparent in the BRE clade of EHB.IMPORTANCE Bacteria living within fungal hyphae present an example of one of the most intimate relationships between fungi and bacteria. Even though there are several well-described examples of such partnerships, their prevalence within the fungal kingdom remains unknown. Our study focused on early divergent terrestrial fungi in the phylum Mucoromycota. We found that ca. 20% of the strains tested harbored bacteria from the family Burkholderiaceae. Not only did we confirm the presence of bacteria from previously described endosymbiont clades, we also identified a new group of endohyphal Burkholderiaceae representing the genus Paraburkholderia. We established that more than half of the screened Umbelopsis strains were positive for bacteria from this new group. We also determined that, while previously described BRE codiverged with their fungal hosts, Paraburkholderia symbionts did not.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Haelewaterse01458-19,

title = {Draft Genome Sequence of the Globally Distributed Cockroach-Infecting Fungus Herpomyces periplanetae Strain D. Haelew. 1187d},

author = {Danny Haelewaters and Alicja Okrasińska and Michał Gorczak and Donald H. Pfister},

editor = {Antonis Rokas},

url = {https://mra.asm.org/content/9/6/e01458-19},

doi = {10.1128/MRA.01458-19},

year  = {2020},

date = {2020-01-01},

journal = {Microbiology Resource Announcements},

volume = {9},

number = {6},

publisher = {American Society for Microbiology Journals},

abstract = {Herpomyces periplanetae is an obligate biotroph of Periplaneta americana, the American cockroach. Its nearly cosmopolitan distribution is shaped by its globally invasive host and the international pet trade. Here, we report the draft genome sequence of H. periplanetae, based on a thallus from P. americana collected in Cambridge, Massachusetts.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{pawlowska2019,

title = {Carbon assimilation profiles of mucoralean fungi show their metabolic versatility},

author = {Julia Pawłowska and Alicja Okrasińska and Kamil Kisło and Tamara Aleksandrzak-Piekarczyk and Katarzyna Szatraj and Somayeh Dolatabadi and Anna Muszewska },

url = {https://www.nature.com/articles/s41598-019-48296-w#citeas},

doi = {https://doi.org/10.1038/s41598-019-48296-w},

year  = {2019},

date = {2019-08-14},

journal = {Scientific Reports},

volume = {9},

pages = {11864},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{BANASIAK201994,

title = {Aureoboletus projectellus (Fungi, Boletales) – An American bolete rapidly spreading in Europe as a new model species for studying expansion of macrofungi},

author = {Łukasz Banasiak and Marcin Pietras and Marta Wrzosek and Alicja Okrasińska and Michał Gorczak and Marta Kolanowska and Julia Pawłowska},

url = {http://www.sciencedirect.com/science/article/pii/S1754504818301491},

doi = {https://doi.org/10.1016/j.funeco.2018.12.006},

issn = {1754-5048},

year  = {2019},

date = {2019-01-01},

journal = {Fungal Ecology},

volume = {39},

pages = {94 - 99},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{4ed53db950814a7ebd07f50586eb547b,

title = {Notes for genera: basal clades of Fungi (including Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Calcarisporiellomycota, Caulochytriomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota, Mucoromycota, Neocallimastigomycota, Olpidiomycota, Rozellomycota and Zoopagomycota)},

author = {Nalin N. Wijayawardene and Julia Pawłowska and Peter M. Letcher and Paul M. Kirk and Richard A. Humber and Arthur Schüßer and Marta Wrzosek and Anna Muszewska and Alicja Okrasińska and Łukasz Istel and Aleksandra Gęsiorska and Paul Mungai and Adebola Azeez Lateef and Kunhiraman C. Rajeshkumar and Rajshree V. Singh and Renate Radek and Grit Walther and Lysett Wagner and Christopher Walker and Siril D. A. Wijesundara and Moslem Papizadeh and Somayeh Dolatabadi and Belle D. Shenoy and Yuri S. Tokarev and Saisamorn Lumyong and Kevin D. Hyde},

doi = {10.1007/s13225-018-0409-5},

issn = {1560-2745},

year  = {2018},

date = {2018-01-01},

journal = {Fungal Diversity},

volume = {92},

number = {1},

pages = {43--129},

publisher = {Springer},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{WRZOSEK2017134,

title = {The progressive spread of Aureoboletus projectellus (Fungi, Basidiomycota) in Europe},

author = {Marta Wrzosek and Jurga Motiejūnaitė and Jonas Kasparavičius and Mateusz Wilk and Edgars Mukins and Jürgen Schreiner and Mikhail Vishnevskiy and Michał Gorczak and Alicja Okrasińska and Łukasz Istel and Julia Pawłowska},

url = {http://www.sciencedirect.com/science/article/pii/S175450481730020X},

doi = {https://doi.org/10.1016/j.funeco.2017.02.003},

issn = {1754-5048},

year  = {2017},

date = {2017-01-01},

journal = {Fungal Ecology},

volume = {27},

pages = {134 - 136},

abstract = {The American bolete Aureoboletus projectellus was first reported in Europe in the first decade of this century. At the beginning, it was only observed on the Baltic Sea shore. Since 2014, the range of this fungus has been significantly expanding, at present it reaches more than 150 km inland and encompasses five countries. Recently, mass occurrences of its fruit bodies were observed in Latvia, Lithuania, and Poland. A. projectellus forms mycorrhizas with native European trees in the range of their natural distribution (Pinus sylvestris) as well as in the areas where they were introduced (Pinus mugo).},

keywords = {},

pubstate = {published},

tppubtype = {article}

}